Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus

Amine Bazaa 1 Paula Juarez 2 Neziha Marrakchi 1 Zakaria Bel Lasfer 1 Mohamed El Ayeb 1 Robert A. Harrison 3 Juan J. Calvete 2 Libia Sanz 2
1 Laboratoire des Venins et Toxines, Institut Pasteur de Tunis
2 IBV - Instituto de biomedicina [Valencia]
3 Alistair Reid Venom Research Unit
Abstract : Analysis of cDNAs from Macrovipera lebetina transmediterranea (Mlt) and Echis ocellatus (Eo) venom gland libraries encoding disintegrins argued strongly for a common ancestry of the messengers of short disintegrins and those for precursors of dimeric disintegrin chains. We now report the sequence analysis of disintegrin-coding genes from these two vipers. Genomic DNAs for dimeric disintegrin subunits Ml_G1 and Ml_G2 (Mlt) and Eo_D3 ( Eo) contain single 1-kb introns exhibiting the 5'-GTAAG (donor)/3'-AG ( acceptor) consensus intron splicing signature. On the other hand, the short RTS-disintegrins M1_G3 ( Mlt) and Eo_RTS ( Eo) and the short RGD-disintegrin ocellatusin (Eo) are transcribed from intronless genomic DNA sequences, indicating that the evolutionary pathway leading to the emergence of short disintegrins involved the removal of all intronic sequences. The insertion position of the intron within Ml_G1, Ml_G2, and Eo_D3 is conserved in the genes for vertebrate ADAM ( A disintegrin and metalloproteinase) protein disintegrinlike domains and within the gene for the medium-size snake disintegrins halystatins 2 and 3. However, a comparative analysis of currently available disintegrin(-like) genes outlines the view that a minimization of both the gene organization and the protein structure underlies the evolution of the snake venom disintegrin family.
Keywords : intronless gene intron sequence genomic DNA disintegrin evolution Echis ocellatus Macrovipera lebetina transmediterranea
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Journal articles
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Submitted on : Monday, September 18, 2017 - 3:57:53 PM
Last modification on : Monday, July 8, 2019 - 4:48:02 PM

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Amine Bazaa, Paula Juarez, Neziha Marrakchi, Zakaria Bel Lasfer, Mohamed El Ayeb, et al.. Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus. Journal of Molecular Evolution, Springer Verlag, 2007, 64 (2), pp.261-271. ⟨10.1007/s00239-006-0161-4⟩. ⟨pasteur-01375230⟩

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