Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

B. Ben Abdelmoumen Mardassi (1) , A. Béjaoui Khiari (1) , L. Oussaief (1) , A. Landoulsi (1) , C. Brik (1) , B. Mlik (1) , F. Amouna (1)

1 Laboratoire des Mycoplasmes

A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-aminoacid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM. (c) 2006 Elsevier B.V. All rights reserved.

Mots clés: fusion protein; species-specific epitopes; Mycoplasma meleagridis; genomic library; serodiagnosis

Domaines: Sciences du Vivant [q-bio]; Mycologie; Biologie cellulaire

B. Ben Abdelmoumen Mardassi, A. Béjaoui Khiari, L. Oussaief, A. Landoulsi, C. Brik, et al.. Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential. Veterinary Microbiology, 2007, 119 (1), pp.31 - 41. ⟨10.1016/j.vetmic.2006.08.008⟩. ⟨pasteur-01375241⟩