Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

B. Ben Abdelmoumen Mardassi 1, * A. Béjaoui Khiari 1 L. Oussaief 1 A. Landoulsi 1 C. Brik 1 B. Mlik 1 F. Amouna 1
* Auteur correspondant
1 Laboratoire des Mycoplasmes
Abstract : A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-aminoacid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM. (c) 2006 Elsevier B.V. All rights reserved.
Keywords : fusion protein species-specific epitopes Mycoplasma meleagridis genomic library serodiagnosis
Type de document :
Article dans une revue
Veterinary Microbiology, Elsevier, 2007, 119 (1), pp.31 - 41. 〈10.1016/j.vetmic.2006.08.008〉
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Soumis le : mardi 2 mai 2017 - 12:38:41
Dernière modification le : lundi 5 février 2018 - 15:22:13

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B. Ben Abdelmoumen Mardassi, A. Béjaoui Khiari, L. Oussaief, A. Landoulsi, C. Brik, et al.. Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential. Veterinary Microbiology, Elsevier, 2007, 119 (1), pp.31 - 41. 〈10.1016/j.vetmic.2006.08.008〉. 〈pasteur-01375241〉

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