Comparison of pp65 antigenemia, quantitative PCR and DNA hybrid capture for detection of cytomegalovirus in transplant recipients and AIDS patients

Leila Mhiri 1 Belhassen Kaabi 2 Mehdi Houimel 3 Zakia Arrouji 1 Amine Slim 1
1 Laboratory of Virology
2 Laboratoire d'Epidémiologie et d'Ecologie parasitaire, Institut Pasteur de Tunis
3 LVGM - Laboratoire d'Immunopathologie, Vaccinologie et Génétique Moléculaire
Abstract : The cytomegalovirus (CMV) antigenemia assay has been used frequently for rapid diagnosis of CMV infection, and antigenemia threshold values are recommended for triggering preemptive therapy. Hybrid capture of CMV's DNA and quantitative polymerase chain reaction (qPCR) are increasingly being adopted for early detection of CMV. The performance of the antigenemia assay, qPCR in plasma and hybrid capture in leukocytes were compared in 110 immunocompromised patients (38 bone-marrow transplants, 50 renal transplants and 22 AIDS patients). The most sensitive test was hybrid capture for transplants, while antigenemia and the qPCR showed similar performance for patients with AIDS. QPCR and hybrid capture thresholds requiring antiviral therapy were calculated using a receiver-operating-characteristic curve for antigenemia values corresponding to 2 positive cells for bone-marrow transplants and to 10 positive cells for renal transplants and AIDS patients. These threshold values varied with the group of patients considered, with corresponding sensitivities higher than 86% and specificities higher than 76% for hybrid capture, and sensitivities higher than 61% and specificities higher than 75% for qPCR in plasma. Hybrid capture in leukocytes can substitute for antigenemia in the case of transplants, and qPCR in plasma can substitute for it in the case of AIDS patients. (C) 2007 Elsevier B.V. All rights reserved.
Keywords : cytomegalovirus hybrid capture qPCR antigenemia
Type de document :
Article dans une revue
Journal of Virological Methods, Elsevier, 2007, 143 (1), pp.23-28. 〈10.1016/j.jviromet.2007.01.033〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01375251
Contributeur : Institut Pasteur Tunis <>
Soumis le : lundi 19 décembre 2016 - 10:16:14
Dernière modification le : lundi 5 février 2018 - 15:22:12

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Leila Mhiri, Belhassen Kaabi, Mehdi Houimel, Zakia Arrouji, Amine Slim. Comparison of pp65 antigenemia, quantitative PCR and DNA hybrid capture for detection of cytomegalovirus in transplant recipients and AIDS patients. Journal of Virological Methods, Elsevier, 2007, 143 (1), pp.23-28. 〈10.1016/j.jviromet.2007.01.033〉. 〈pasteur-01375251〉

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