Development of a real-time PCR for tomato yellow leaf curl Sardinia virus

S. Gharsallah Chouchane (1) , F. Gorsane (1, 2) , H. Louzir (3) , M. K. Nakhla (4) , M. Marrakchi (1) , H. Fakhfakh (1, 2)

1 FST - Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis
2 Faculté des Sciences de Bizerte [Université de Carthage]
3 Laboratoire d'Immunologie, Institut Pasteur de Tunis
4 University of Wisconsin-Madison

Recently, tomato yellow leaf curl disease has become important for the tomato grown both in greenhouse and field conditions in Tunisia. Here, we describe a rapid, specific, reliable, and sensitive real-time PCR, based on TaqMan (R) chemistry, for Tomato yellow leaf curl Sardinia virus (TYLCSV). This method proved suitable for the detection and quantification of this virus in tomato, pepper and bean plants. It detected the virus even in the samples that were negative by conventional assays.

Keywords: coat-protein gene; SYBR (R) Green I chemistry; real-time PCR; TaqMan (R) chemistry; tomato yellow leaf curl Sardinia virus

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Vegetal Biology; Botanics

S. Gharsallah Chouchane, F. Gorsane, H. Louzir, M. K. Nakhla, M. Marrakchi, et al.. Development of a real-time PCR for tomato yellow leaf curl Sardinia virus. Acta Virologica, 2006, 50 (2), pp.101-105. ⟨pasteur-01375257⟩