Expression colique de l’interféron-γ et de l’interleukine-10 au cours de la maladie de Crohn et de la rectocolite ulcéro-hémorragique

L. Kallel 1 J. Boubaker 1, * H. Louzir 2 M. Ben Ahmed 2 A. Sassi S. Boubaker 3 B. Zouari 4 A. Filali 1
* Auteur correspondant
1 Service de gastroentérologie A
2 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections
3 Institut Pasteur de Tunis
4 Département de médecine préventive et de statistiques
Résumé : The aim of this study was to investigate mucosal expression of INF-gamma and IL-10 in patients with Crohn's disease (CD) or ulcerative colitis (UC).Fourteen patients with CD and 11 patients with ulcerative colitis participated and 7 healthy subjects were also included. Study of the mucosal expression of INF-gamma and IL-10 was conducted using biopsies from healthy and damaged colons, using the inverse transcription and genetic amplification (RT-PCR) technique in real time (Taqman). Our results were expressed as ratio between messenger cytokine (mRNA) levels and ribosomal RNA level of a reference molecule (rRNA 18S), then multiplied by 108.In the cases of Crohn's disease, the mucosa expressed increased INF-gamma and IL-10 compared with controls (respective medians: 23.03 vs. 1.87 p=0.04 and 20.61 vs. 2.13 p=0.08). A strong positive correlation was found in the mucosal expression of IL-10 and INF-gamma during CD (r=0.9 p<0.0001). In contrast, in patients with UC, the expression of INF-gamma and IL-10 were comparable to those observed in the controls (7.18 vs. 2.18 p=0.36 and 3.66 vs. 1.87 p=0.44).During Crohn's disease, the expression of both IL-10 and INF-gamma was increased and strongly correlated, compared with the controls.
Type de document :
Article dans une revue
La Presse Médicale, Elsevier Masson, 2005, 34 (1), pp.8 - 12. 〈10.1016/S0755-4982(05)83876-X〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01375269
Contributeur : Institut Pasteur Tunis <>
Soumis le : mardi 20 décembre 2016 - 09:44:10
Dernière modification le : jeudi 7 juin 2018 - 14:54:03

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L. Kallel, J. Boubaker, H. Louzir, M. Ben Ahmed, A. Sassi, et al.. Expression colique de l’interféron-γ et de l’interleukine-10 au cours de la maladie de Crohn et de la rectocolite ulcéro-hémorragique. La Presse Médicale, Elsevier Masson, 2005, 34 (1), pp.8 - 12. 〈10.1016/S0755-4982(05)83876-X〉. 〈pasteur-01375269〉

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