In Vivo Prevention of Bladder Urotoxicity: Purified Hydroxytyrosol Ameliorates Urotoxic Effects of Cyclophosphamide and Buthionine Sulfoximine in Mice

Kamel Rouissi 1 Bechr Hamrita 1 Soumaya Kouidi 1 Yosra Messai 1 Bassem Jaouadi 2 Imen Medimegh 1 Khaled Hamden 3 Slah Ouerhani 4 Mohamed Cherif 5 Amel Benammar Elgaaied 1
1 Laboratory of Genetics, Immunology and Human Pathology
2 CBS - Centre de Biotechnologie de Sfax
3 Lab Anim Ecophysiol
4 Institut Pasteur de Tunis
5 Department of Urology
Abstract : Urotoxicity is a troublesome complication associated with cyclophosphamide (CP) and L-buthionine-SR-sulfoximine (BSO) treatment in chemotherapy. With this concern in mind, the present study investigated the potential effects of a hydroxytyrosol extract from olive mill waste (OMW) on urotoxicity induced by acute CP and BSO doses using a Swiss albino mouse model. Toxicity modulation was evaluated by measuring lipid peroxidation (LPO) and antioxidants in urinary bladder. The findings revealed that the hydroxytyrosol extract exerted a protective effect not only on LPO but also on enzymatic antioxidants. When compared to the controls, the CP-treated animals underwent significant decreases in the glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GP), and catalase (CAT) activities. The level of glutathione (GSH) was also reduced with increased doses of LPO in the CP-treated animals. L-Buthionine-SR-sulfoximine treatment exerted an additive toxic effect on the CP-treated animals. Interestingly, pretreatment with the hydroxytyrosol extract restored the activities of all enzymes back to normal levels and exhibited an overall protective effect on the CP- and BSO-induced toxicities in urinary bladder. The restoration of GSH through the treatment with the hydroxytyrosol extract can play an important role in reversing CP-induced apoptosis and free radical-mediated LPO.
Keywords : antioxidants hydroxytyrosol olive mill waste polyphenols cyclophosphamide BSO urotoxicity
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Article dans une revue
International Journal of Toxicology, SAGE Publications, 2011, 30 (4), pp.419-427. 〈10.1177/1091581811410444〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01375284
Contributeur : Institut Pasteur Tunis <>
Soumis le : mardi 20 décembre 2016 - 15:50:29
Dernière modification le : mardi 15 mai 2018 - 15:22:39

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Kamel Rouissi, Bechr Hamrita, Soumaya Kouidi, Yosra Messai, Bassem Jaouadi, et al.. In Vivo Prevention of Bladder Urotoxicity: Purified Hydroxytyrosol Ameliorates Urotoxic Effects of Cyclophosphamide and Buthionine Sulfoximine in Mice. International Journal of Toxicology, SAGE Publications, 2011, 30 (4), pp.419-427. 〈10.1177/1091581811410444〉. 〈pasteur-01375284〉

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