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In Vivo Prevention of Bladder Urotoxicity: Purified Hydroxytyrosol Ameliorates Urotoxic Effects of Cyclophosphamide and Buthionine Sulfoximine in Mice

Abstract : Urotoxicity is a troublesome complication associated with cyclophosphamide (CP) and L-buthionine-SR-sulfoximine (BSO) treatment in chemotherapy. With this concern in mind, the present study investigated the potential effects of a hydroxytyrosol extract from olive mill waste (OMW) on urotoxicity induced by acute CP and BSO doses using a Swiss albino mouse model. Toxicity modulation was evaluated by measuring lipid peroxidation (LPO) and antioxidants in urinary bladder. The findings revealed that the hydroxytyrosol extract exerted a protective effect not only on LPO but also on enzymatic antioxidants. When compared to the controls, the CP-treated animals underwent significant decreases in the glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GP), and catalase (CAT) activities. The level of glutathione (GSH) was also reduced with increased doses of LPO in the CP-treated animals. L-Buthionine-SR-sulfoximine treatment exerted an additive toxic effect on the CP-treated animals. Interestingly, pretreatment with the hydroxytyrosol extract restored the activities of all enzymes back to normal levels and exhibited an overall protective effect on the CP- and BSO-induced toxicities in urinary bladder. The restoration of GSH through the treatment with the hydroxytyrosol extract can play an important role in reversing CP-induced apoptosis and free radical-mediated LPO.
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https://hal-riip.archives-ouvertes.fr/pasteur-01375284
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Submitted on : Tuesday, December 20, 2016 - 3:50:29 PM
Last modification on : Wednesday, October 28, 2020 - 9:52:04 AM

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Kamel Rouissi, Bechr Hamrita, Soumaya Kouidi, yosra Messai, Bassem Jaouadi, et al.. In Vivo Prevention of Bladder Urotoxicity: Purified Hydroxytyrosol Ameliorates Urotoxic Effects of Cyclophosphamide and Buthionine Sulfoximine in Mice. International Journal of Toxicology, SAGE Publications, 2011, 30 (4), pp.419-427. ⟨10.1177/1091581811410444⟩. ⟨pasteur-01375284⟩

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