Lack of effect of tumor necrosis factor-alpha-308 G/A polymorphism on severity of liver fibrosis in Tunisian hepatitis C virus (HCV)-infected patients

N. Bouzgarrou 1 E. Hassen 1 S. Gabbouj 1 E. Schvoerer 2 N. Ben Mami 3 H. Triki 4 L. Chouchane 5
1 Laboratoire de Physiologie, Faculté de médecine de Monastir, Université de Monastir, Tunisie
2 Institut de Virologie
3 Gastroenterology B Unit
4 Laboratoire de Virologie Clinique, Référence Régional OMS pour la Poliomyélite et la Rougeole - Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles
5 Genetic Medicine Department
Abstract : Objectives. - Tumor necrosis factor alpha (INF-alpha) plays a key role in the immune response. An elevated plasma level of INF-alpha was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-alpha expression. In this study, we aimed to evaluate the impact of TNF-alpha -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. Methods. - TNF-alpha -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). Results. - The genotype distribution of the INF-alpha -308 G/A polymorphism among the HCV-infected patients was as follows : GG : 67.9%, GA: 32.1%, AA: 0%. With regard to fibrosis score, no significant differences in TNF-alpha genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15) Conclusion. - No significant association between TNF-alpha -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.
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Gastroentérologie Clinique et Biologique / Research and Clinics in Hepatology and Gastroenterology, Elsevier Masson, 2010, 34 (4-5), pp.297-304. 〈10.1016/j.gcb.2010.03.008〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01375285
Contributeur : Institut Pasteur Tunis <>
Soumis le : mercredi 21 décembre 2016 - 09:24:45
Dernière modification le : lundi 8 octobre 2018 - 17:44:06

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N. Bouzgarrou, E. Hassen, S. Gabbouj, E. Schvoerer, N. Ben Mami, et al.. Lack of effect of tumor necrosis factor-alpha-308 G/A polymorphism on severity of liver fibrosis in Tunisian hepatitis C virus (HCV)-infected patients. Gastroentérologie Clinique et Biologique / Research and Clinics in Hepatology and Gastroenterology, Elsevier Masson, 2010, 34 (4-5), pp.297-304. 〈10.1016/j.gcb.2010.03.008〉. 〈pasteur-01375285〉

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