Expression of WISP3 and RhoC genes at mRNA and protein levels in inflammatory and noninflammatory breast cancer in Tunisian patients.

Raja Marrakchi 1 Ines Khadimallah 1 Slah Ouerhani 2 Amor Gamoudi 3 Fathi Khomsi 4 Hatem Bouzaine 4 Mohamed Benamor 3 Karim Bougatef 1 Samia Mnif 4 Rayhana Zitoun 5 Farouk Benna 3 Hammouda Boussen 3 Khaled Rahal Amel Benammar Elgaaied 6
1 Laboratory of Genetics, Immunology and Human Pathology
2 Institut Pasteur de Tunis
3 Inst Carcinol Salah Azaiz Tunis
4 LR11IPT07 - Laboratoire d'hématologie moléculaire et cellulaire
5 PPRIME - Institut Pprime
6 UTM - Université Tunis El Manar
Abstract : Previous studies have shown the expression WISP3 and RhoC in cell lines of inflammatory breast cancer (IBC). The aim in the current study was to compare the expression of both genes, in biopsy samples collected from Tunisian patients with localized or metastatic breast cancer and patients with IBC. We investigated 127 patients enrolled in Salah Azaiez Institute in Tunis. Using the RT-PCR, we showed the phenotype (WISP3-, RhoC+) is significantly associated with IBC tumors, while the (WISP3+, RhoC-)phenotype is mostly associated to non-IBC tumors. The frequencies of these tumor phenotypes are significantly different between these tumor groups (p = 10(- 7); relative risk or RR = 3.25; confidential interval or CI 95% = 1.90-5.53). Immunohistochemical test revealing the presence of WISP3 and RhoC proteins correlates with the expression in the biopsy of their encoding genes as detected by RT-PCR. In conclusion, it appears that WISP3 and RhoC genes expression status defines a molecular signature of IBC.
Keywords : WISP3 RT-PCR Inflammatory breast cancer Immunohistochemistry Tunisian population RhoC
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Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-01375289
Contributor : Institut Pasteur Tunis <>
Submitted on : Wednesday, December 21, 2016 - 11:18:36 AM
Last modification on : Tuesday, June 4, 2019 - 6:22:16 PM

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RIIP | RIIP_TUNIS | CNRS | UNIV-POITIERS | PPRIME

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Raja Marrakchi, Ines Khadimallah, Slah Ouerhani, Amor Gamoudi, Fathi Khomsi, et al.. Expression of WISP3 and RhoC genes at mRNA and protein levels in inflammatory and noninflammatory breast cancer in Tunisian patients.. Cancer Investigation, Taylor & Francis, 2010, 28 (4), pp.399-407. ⟨10.3109/07357900903405926⟩. ⟨pasteur-01375289⟩

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