Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation.

Fethi Ben Abdallah 1 Ali Ellafi 2 Rihab Lagha 2 Amina Bakhrouf 2 Abdelkader Namane 3 Jean-Claude Rousselle 3 Pascal Lenormand 3 Héla Kallel 4
1 Fermentation et de Développement de Vaccins Virologiques
2 LATVPEP - Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits
3 Protéomique (Plate-Forme)
4 Département de Biologie, Unité de Biochimie des Lipides et des Protéines
Abstract : Vibrio parahaemolyticus and Vibrio alginolyticus were subjected to γ-irradiation (0.5 kGy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. After treatment, outer membrane protein profiles of starved or γ-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Altered proteins were identified by mass spectrometry (MS and MS/MS) and analyses revealed that OmpU can be considered a starvation stress-induced protein. In addition, expression of OtnA, OmpW, OmpA and peptidoglycan-associated lipoprotein decreased to non-detectable levels in starved cells. Furthermore, MltA-interacting protein MipA appeared under γ-irradiation or starvation conditions. Thus, it can be considered to be a γ-irradiation, long-term starvation stress protein in some vibrios.
Keywords : Alteration Outer membrane proteins Mass spectrometry Vibrio Starvation gamma-Irradiation
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Journal articles
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Submitted on : Tuesday, January 3, 2017 - 3:04:45 PM
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Fethi Ben Abdallah, Ali Ellafi, Rihab Lagha, Amina Bakhrouf, Abdelkader Namane, et al.. Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation.. Research in Microbiology, Elsevier, 2010, 161 (10), pp.869-75. ⟨10.1016/j.resmic.2010.10.009⟩. ⟨pasteur-01375303⟩

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