Association of HLA-G+3142 C > G polymorphism and breast cancer in Tunisian population

Abstract : HLA-G is highly expressed in cancer. Also, it is associated to its progression. Here, we explored the relationship between two HLA-G polymorphisms with breast cancer (BC) and tried to make a correlation with sHLA-G levels. We genotyped 104 patients with BC and 83 controls (CTRL) for HLA-G 14-bp insertion/deletion (Ins/Del) and HLA-G +3142 C > G polymorphisms. The mutations were identified with PCR and PCR-RFLP. The sHLA-G dosage was performed on plasma samples by a specific ELISA. A significant association with BC was found concerning the G allele in the +3142 C > G polymorphism (p = 0.0004). The G/G genotype is the protective genotype (1 % in BC patients vs. 13.1 % in CTRL, OR 0.065, 95 % CI 0.008-0.523). No statistically significant differences were observed for the 14-bp Ins/Del polymorphism between BC patients and controls frequencies. The protection by G/G genotype of +3142 C > G polymorphism is maintained in young patients (< 50 years, p = 0.0006) and in early-diagnosed BC patients (< 50 years, p = 0.0033). In addition, an association was found between the haplotypes inferred by both HLA-G polymorphisms and BC susceptibility. Indeed, the (DelG) haplotype is found as the protective haplotype against BC (OR 0.269, 95 % CI 0.081-0.895, p = 0.023). The ELISA dosage of sHLA-G revealed increased levels in BC compared to CTRL (p < 0.0001). We demonstrated also that sHLA-G is closely associated with advanced stages of BC without significance. sHLA-G is increased in TNM IV and SBR III subgroups. It is also enhanced in patients with a tumor size over 20 mm and in triple-negative patients. Taken together, our findings demonstrate, for the first time, the association of HLA-G +3142 C > G polymorphism with BC susceptibility in Tunisian population. Our results revealed also a potential implication of sHLA-G in advanced stages of BC.
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Immunologic Research, Humana Press, 2016, 〈10.1007/s12026-015-8782-6 〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01400585
Contributeur : Institut Pasteur Tunis <>
Soumis le : mardi 29 novembre 2016 - 10:52:34
Dernière modification le : lundi 8 octobre 2018 - 17:44:07

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Inès Zidi, Olfa Dziri, Nour Zidi, Refaat Sebai, Nadia Boujelebene, et al.. Association of HLA-G+3142 C > G polymorphism and breast cancer in Tunisian population. Immunologic Research, Humana Press, 2016, 〈10.1007/s12026-015-8782-6 〉. 〈pasteur-01400585〉

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