Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity

Ülkü Anik (1) , Yudum Tepeli (1) , Mohamed F. Diouani (2, 3)

1 Mugla Sitki Kocman University
2 Institut Pasteur de Tunis
3 UTM - Université de Tunis El Manar

Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor's working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscopy

Domains: Life Sciences [q-bio]; Chemical Sciences

Ülkü Anik, Yudum Tepeli, Mohamed F. Diouani. Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity. Analytical Chemistry, 2016, 88 (12), pp.6151 - 6153. ⟨10.1021/acs.analchem.6b01720⟩. ⟨pasteur-01405788⟩