Antibacterial and Antifungal Activities of Ethanol Extracts of Halimium halimifolium, Cistus salviifolius and Cistus monspeliensis

Abstract : The objective of this study was to evaluate antimicrobial and antifungal activities of crude extracts from leaves and flower of Halimium halimifolium, and compared with those of Cistus salviifolius and Cistus monspeliensis. The tested plants (leaves and flowers) were extracted with ethanol, the activities were screened against three Gram-positive (Listeria monocytogenes, Bacillus subtilis and staphylococcus aureus), three Gram-negative bacteria (Salmonella enteric, Pseudomonas aeruginosa and Escherichia coli), and two pathogenic fungi (Candida albicans and Aspergillus niger). The efficacy of these extracts was tested against those microorganisms through a disc-diffusion method employing 15 í µí¼‡L of each sample per paper discs (6 mm in diameter). Comparable results were carried out using Gentamicin and Amphotericin as standard antibiotics. Ethanol extracts of different parts of plant exhibited good activity against all microorganisms tested. The inhibition zone measured ranged from 10 to 26 mm against all the bacteria and 8 to 20 mm against fungal strains. The minimum inhibitory and bactericidal concentrations (MIC and MBC) values against Staphylococcus aureus ranged from 1.562 to 12.5 mg. mL-1 and 3.125 to 25 mg. mL-1 respectively. The Cistus salviifolius leaves had the most potent bactericidal activity. On the other hand, Candida albicans and Aspergillus Niger were the least susceptible microorganisms to all Cistus extracts. In the present work, the antimicrobial potential of ethanol extracts of leaves and flower of Halimium halimifolium, Cistus salviifolius and Cistus monspeliensis is demonstrated. The high levels of antibacterial activities of have been detected, indicating that this plant may serve as an excellent source of natural antibacterial for disease prevention.
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International Journal of Pharmaceutical and Clinical Research, 2016, 8, pp.243 - 247
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Ahlem Rebaya, Igueld Souad, Safa Hammrouni, Abderrazak Maaroufi, Malika Ayadi, et al.. Antibacterial and Antifungal Activities of Ethanol Extracts of Halimium halimifolium, Cistus salviifolius and Cistus monspeliensis. International Journal of Pharmaceutical and Clinical Research, 2016, 8, pp.243 - 247. 〈pasteur-01448107〉

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