Evaluation of anti-proliferative and anti-inflammatory activities of Pelagia noctiluca venom in Lipopolysaccharide/Interferon-γ stimulated RAW264.7 macrophages

Yosra Ayed (1, 2) , Rabiaa Manel Sghaier (3, 4) , Dhafer Laouini (3) , Hassen Bacha (2, 5)

1 Taibah University
2 FMDM - Faculté de Médecine Dentaire de Monastir [Tunisie]
3 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection
4 UTM - Université de Tunis El Manar
5 UJ - Université de Jendouba

Components of Pelagia noctiluca (P. noctiluca) venom were evaluated for their anticancer and nitric Oxide (NO) inhibition activities. Three fractions, out of four, obtained by gel filtration on Sephadex G75 of P. noctiluca venom revealed an important selective anti-proliferative activity on several cell lines such as human bladder carcinoma (RT112), human glioblastoma (U87), and human myelogenous leukemia (K562) but not on mitogen-stimulated peripheral blood mononuclear cells. Interestingly, P. noctiluca components showed an important dose-dependent anti-inflammatory activity, through inhibition of NO production via transcriptional regulation of Inducible NO Synthase (iNOS), in IFN-γ/LPS stimulated RAW 264.7 macrophages. These data strongly suggest that P. noctiluca venom could be used as a natural inhibitor of cancer cell lines and a potent anti-inflammatory agent for the treatment of anti-inflammatory diseases.

Keywords: Anti-inflammatory activity; Cell proliferation; MTT assay; Nitric oxide; Pelagia noctiluca; Venom

Domains: Life Sciences [q-bio]; Pharmaceutical sciences

Yosra Ayed, Rabiaa Manel Sghaier, Dhafer Laouini, Hassen Bacha. Evaluation of anti-proliferative and anti-inflammatory activities of Pelagia noctiluca venom in Lipopolysaccharide/Interferon-γ stimulated RAW264.7 macrophages. Biomedicine and Pharmacotherapy, 2016, 84, pp.1986 - 1991. ⟨10.1016/j.biopha.2016.11.010⟩. ⟨pasteur-01455071⟩