Structural characterization and biological implications of sulfated N-glycans in a serine protease from the neotropical moth Hylesia metabus (Cramer [1775]) (Lepidoptera: Saturniidae).

Abstract : Contact with the urticating setae from the abdomen of adult females of the neo-tropical moth Hylesia metabus gives rise to an urticating dermatitis, characterized by intense pruritus, generalized malaise and occasionally ocular lesions (lepidopterism). The setae contain a pro-inflammatory glycosylated protease homologous to other S1A serine proteases of insects. Deglycosylation with PNGase F in the presence of a buffer prepared with 40% H2 (18)O allowed the assignment of an N-glycosylation site. Five main paucimannosidic N-glycans were identified, three of which were exclusively α(1-6)-fucosylated at the proximal GlcNAc. A considerable portion of these N-glycans are anionic species sulfated on either the 4- or the 6-position of the α(1-6)-mannose residue of the core. The application of chemically and enzymatically modified variants of the toxin in an animal model in guinea pigs showed that the pro-inflammatory and immunological reactions, e.g. disseminated fibrin deposition and activation of neutrophils, are due to the presence of sulfate-linked groups and not on disulfide bonds, as demonstrated by the reduction and S-alkylation of the toxin. On the other hand, the hemorrhagic vascular lesions observed are attributed to the proteolytic activity of the toxin. Thus, N-glycan sulfation may constitute a defense mechanism against predators.
Type de document :
Article dans une revue
Glycobiology, Oxford University Press (OUP), 2015, pp.230-50
Liste complète des métadonnées

https://hal-riip.archives-ouvertes.fr/pasteur-01498327
Contributeur : Mariella Botta <>
Soumis le : mercredi 29 mars 2017 - 19:14:12
Dernière modification le : jeudi 20 septembre 2018 - 13:56:06

Identifiants

  • HAL Id : pasteur-01498327, version 1
  • PUBMED : 26537504

Collections

Citation

Gleysin Cabrera, Víctor Salazar, Raquel Montesino, Yanet Támbara, Weston B Struwe, et al.. Structural characterization and biological implications of sulfated N-glycans in a serine protease from the neotropical moth Hylesia metabus (Cramer [1775]) (Lepidoptera: Saturniidae).. Glycobiology, Oxford University Press (OUP), 2015, pp.230-50. 〈pasteur-01498327〉

Partager

Métriques

Consultations de la notice

22