Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.

Abstract : In this note, we present a discussion of the advantages and scope of model-free analysis methods applied to the popular solvatochromic probe LAURDAN, which is widely used as an environmental probe to study dynamics and structure in membranes. In particular, we compare and contrast the generalized polarization approach with the spectral phasor approach. To illustrate our points we utilize several model membrane systems containing pure lipid phases and, in some cases, cholesterol or surfactants. We demonstrate that the spectral phasor method offers definitive advantages in the case of complex systems.
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Methods and Applications in Fluorescence, 2015, 3 (4), 〈10.1088/2050-6120/3/4/047001〉
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Soumis le : mercredi 29 mars 2017 - 21:06:30
Dernière modification le : lundi 12 mars 2018 - 15:40:02

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Leonel Malacrida, Enrico Gratton, David M Jameson. Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.. Methods and Applications in Fluorescence, 2015, 3 (4), 〈10.1088/2050-6120/3/4/047001〉. 〈pasteur-01498362〉

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