High level of IL-10 expression in the blood of animal models possibly relates to resistance against leptospirosis

Mariko Matsui (1) , Roche Louise (1) , Soupé-Gilbert Marie-Estelle (1) , Milena Hasan (2) , Didier Monchy (3) , Cyrille Goarant (1)

1 Institut Pasteur de Nouvelle-Calédonie
2 CRT - Centre de Recherche Translationnelle - Center for Translational Science
3 Centre hospitalier territorial Gaston-Bourret [Nouméa]

Leptospirosis is a severe zoonosis which immunopathogenesis is poorly understood. We evaluated correlation between acute form of the disease and the ratio of the anti-inflammatory cytokine IL-10 to the pro-inflammatory TNF-α and IL-1β expression during the early phase of infection comparing resistant mice and susceptible hamsters infected with two different species of virulent Leptospira. The IL-10/TNF-α and IL-10/IL-1β expression ratios were higher in mouse compared to hamster independently of the Leptospira strain, suggesting a preponderant role of the host response and notably these cytokines in the clinical expression and survival to leptospirosis. Using an IL-10 neutralization strategy in Leptospira-infected mouse model, we also showed evidence of a possible role of this cytokine on host susceptibility, bacterial clearance and on regulation of cytokine gene expression.

Mots clés: IL-10; Cytokine; In vivo; IL-1β; TNF-α; Leptospirosis

Domaines: Maladies infectieuses; Immunité innée; Bactériologie

Mariko Matsui, Roche Louise, Soupé-Gilbert Marie-Estelle, Milena Hasan, Didier Monchy, et al.. High level of IL-10 expression in the blood of animal models possibly relates to resistance against leptospirosis. Cytokine, 2017, 96, pp.144-151. ⟨10.1016/j.cyto.2017.03.009⟩. ⟨pasteur-01510838⟩