Role of Conformational Motions in Enzyme Function: Selected Methodologies and Case Studies.

Abstract : It is now common knowledge that enzymes are mobile entities relying on complex atomic-scale dynamics and coordinated conformational events for proper ligand recognition and catalysis. However, the exact role of protein dynamics in enzyme function remains either poorly understood or difficult to interpret. This mini-review intends to reconcile biophysical observations and biological significance by first describing a number of common experimental and computational methodologies employed to characterize atomic-scale residue motions on various timescales in enzymes, and second by illustrating how the knowledge of these motions can be used to describe the functional behavior of enzymes and even act upon it. Two biologically relevant examples will be highlighted, namely the HIV-1 protease and DNA polymerase β enzyme systems.
Type de document :
Article dans une revue
Catalysts, MDPI, 2017, Special Issue "Enzymes Catalysts", 6 (6), pp.81. 〈10.3390/catal6060081〉
Liste complète des métadonnées

Littérature citée [115 références]  Voir  Masquer  Télécharger

https://hal-riip.archives-ouvertes.fr/pasteur-01534404
Contributeur : Michel Courcelles <>
Soumis le : mercredi 7 juin 2017 - 15:27:30
Dernière modification le : lundi 8 octobre 2018 - 17:44:04

Fichier

nihms852606.pdf
Fichiers produits par l'(les) auteur(s)

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Chitra Narayanan, David N Bernard, Nicolas Doucet. Role of Conformational Motions in Enzyme Function: Selected Methodologies and Case Studies.. Catalysts, MDPI, 2017, Special Issue "Enzymes Catalysts", 6 (6), pp.81. 〈10.3390/catal6060081〉. 〈pasteur-01534404〉

Partager

Métriques

Consultations de la notice

47

Téléchargements de fichiers

33