Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line.

Christine Kleinert 1 Emilie Lacaze 1 Méryl Mounier 1 Sylvain De Guise 2 Michel Fournier 1
1 INRS-IAF - Institut Armand Frappier
2 Department of Pathobiology and Veterinary Science
Abstract : The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500μg/L 17α-ethinyl estradiol and 25,000μg/L naproxen. Exposure to 12,500μg/L 17α-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds.
Keywords : Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals
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Article dans une revue
Marine Pollution Bulletin, Elsevier, 2017, 118 (1-2), pp.237-247. 〈10.1016/j.marpolbul.2017.02.041〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01534638
Contributeur : Michel Courcelles <>
Soumis le : mercredi 7 juin 2017 - 20:10:26
Dernière modification le : lundi 8 octobre 2018 - 17:44:05

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Christine Kleinert, Emilie Lacaze, Méryl Mounier, Sylvain De Guise, Michel Fournier. Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line.. Marine Pollution Bulletin, Elsevier, 2017, 118 (1-2), pp.237-247. 〈10.1016/j.marpolbul.2017.02.041〉. 〈pasteur-01534638〉

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