Skip to Main content Skip to Navigation
New interface
Journal articles

Ultrastructural Characterization of Zika Virus Replication Factories.

Abstract : A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.
Document type :
Journal articles
Complete list of metadata

Cited literature [35 references]  Display  Hide  Download
Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Thursday, June 8, 2017 - 8:19:11 PM
Last modification on : Monday, May 18, 2020 - 11:00:35 AM
Long-term archiving on: : Saturday, September 9, 2017 - 1:46:35 PM


Files produced by the author(s)


Distributed under a Creative Commons Attribution 4.0 International License




Mirko Cortese, Sarah Goellner, Eliana Gisela Acosta, Christopher John Neufeldt, Olga Oleksiuk, et al.. Ultrastructural Characterization of Zika Virus Replication Factories.. Cell Reports, 2017, 18 (9), pp.2113-2123. ⟨10.1016/j.celrep.2017.02.014⟩. ⟨pasteur-01535196⟩



Record views


Files downloads