Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.

Adrien Chauvier 1 Frédéric Picard-Jean 1 Jean-Christophe Berger-Dancause 1 Laurène Bastet 1 Mohammad Reza Naghdi 2 Audrey Dubé 1 Pierre Turcotte 1 Jonathan Perreault 2 Daniel A Lafontaine 1
1 Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke
2 INRS-IAF - Institut Armand Frappier
Abstract : On the basis of nascent transcript sequencing, it has been postulated but never demonstrated that transcriptional pausing at translation start sites is important for gene regulation. Here we show that the Escherichia coli thiamin pyrophosphate (TPP) thiC riboswitch contains a regulatory pause site in the translation initiation region that acts as a checkpoint for thiC expression. By biochemically probing nascent transcription complexes halted at defined positions, we find a narrow transcriptional window for metabolite binding, in which the downstream boundary is delimited by the checkpoint. We show that transcription complexes at the regulatory pause site favour the formation of a riboswitch intramolecular lock that strongly prevents TPP binding. In contrast, cotranscriptional metabolite binding increases RNA polymerase pausing and induces Rho-dependent transcription termination at the checkpoint. Early transcriptional pausing may provide a general mechanism, whereby transient transcriptional windows directly coordinate the sensing of environmental cues and bacterial mRNA regulation.
Type de document :
Article dans une revue
Nature Communications, Nature Publishing Group, 2017, 8, pp.13892. 〈10.1038/ncomms13892〉
Liste complète des métadonnées

Littérature citée [62 références]  Voir  Masquer  Télécharger
https://hal-riip.archives-ouvertes.fr/pasteur-01535405
Contributeur : Michel Courcelles <>
Soumis le : jeudi 8 juin 2017 - 21:06:27
Dernière modification le : lundi 5 février 2018 - 15:22:10
Document(s) archivé(s) le : mercredi 13 décembre 2017 - 08:02:45

Fichier

ncomms13892.pdf
Publication financée par une institution

Identifiants

Collections

RIIP | INRS-IAF

Citation

Adrien Chauvier, Frédéric Picard-Jean, Jean-Christophe Berger-Dancause, Laurène Bastet, Mohammad Reza Naghdi, et al.. Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.. Nature Communications, Nature Publishing Group, 2017, 8, pp.13892. 〈10.1038/ncomms13892〉. 〈pasteur-01535405〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

15

Téléchargements de fichiers

19

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD