Pilot-scale biopesticide production by Bacillus thuringiensis subsp. kurstaki using starch industry wastewater as raw material

Adama Ndao 1, * Balasubramanian Sellamuthu 2 Jean R. Gnepe 1 Rajeshwar D. Tyagi 1 Jose R. Valero 1
* Auteur correspondant
1 INRS-ETE - Eau Terre Environnement - INRS
2 INRS-IAF - Institut Armand Frappier
Abstract : Pilot-scale Bacillus thuringiensis based biopesticide production (2000 L bioreactor) was conducted using starch industry wastewater (SIW) as a raw material using optimized operational parameters obtained in 15 L and 150 L fermenters. In pilot scale fermentation process the oxygen transfer rate is a major limiting factor for high product yield. Thus, the volumetric mass transfer coefficient (KLa) remains a tool to determine the oxygen transfer capacity [oxygen utilization rate (OUR) and oxygen transfer rate (OTR)] to obtain better bacterial growth rate and entomotoxicity in new bioreactor process optimization and scale-up. This study results demonstrated that the oxygen transfer rate in 2000 L bioreactor was better than 15 L and 150 L fermenters. The better oxygen transfer in 2000 L bioreactor augmented the bacterial growth [total cell (TC) and viable spore count (SC)] and delta-endotoxin yield. Prepared a stable biopesticide formulation for field use and its entomotoxicity was also evaluated. This study result corroborates the feasibility of industrial scale operation of biopesticide production using starch industry wastewater as raw material.
Keywords : scale-up Biopesticide formulation KLa bioreactor Bacillus thuringiensis starch industry wastewater
Type de document :
Article dans une revue
Journal of Environmental Science and Health, Part B, Taylor & Francis, 2017, pp.1 - 8. 〈10.1080/03601234.2017.1330071〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01553419
Contributeur : Michel Courcelles <>
Soumis le : lundi 3 juillet 2017 - 15:30:55
Dernière modification le : lundi 5 février 2018 - 15:22:10

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Adama Ndao, Balasubramanian Sellamuthu, Jean R. Gnepe, Rajeshwar D. Tyagi, Jose R. Valero. Pilot-scale biopesticide production by Bacillus thuringiensis subsp. kurstaki using starch industry wastewater as raw material. Journal of Environmental Science and Health, Part B, Taylor & Francis, 2017, pp.1 - 8. 〈10.1080/03601234.2017.1330071〉. 〈pasteur-01553419〉

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