Survey of High-Affinity H2-Oxidizing Bacteria in Soil Reveals Their Vast Diversity Yet Underrepresentation in Genomic Databases.

Abstract : While high-affinity H2-oxidizing bacteria (HA-HOB) serve as the main sink of atmospheric H2, the ecology of this specialist functional group is rather unknown due to its recent discovery. The main purpose of our study is to provide the first extensive survey of HA-HOB in farmland, larch, and poplar soils exposed to 0.5 and 10,000 ppmv H2. Using qPCR and qRT-PCR assays along with PCR amplicon high-throughput sequencing of hhyL gene encoding for the large subunit of high-affinity [NiFe]-hydrogenases (HAH), we found that hhyL gene expression ratio explained better variation in measured H2 oxidation rates than HA-HOB species richness. Carbon, nitrogen, pH, and bacterial species richness appeared as the most important drivers of HA-HOB community structure. Our study also highlights the need to cultivate HA-HOB due to the huge gap in current genomic databases.
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Article dans une revue
Microbial Ecology, Springer Verlag, 2017, 〈10.1007/s00248-017-1011-1〉
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Soumis le : mardi 15 août 2017 - 21:30:13
Dernière modification le : samedi 14 octobre 2017 - 01:03:32

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Sarah Piché-Choquette, Mondher Khdhiri, Philippe Constant. Survey of High-Affinity H2-Oxidizing Bacteria in Soil Reveals Their Vast Diversity Yet Underrepresentation in Genomic Databases.. Microbial Ecology, Springer Verlag, 2017, 〈10.1007/s00248-017-1011-1〉. 〈pasteur-01574608〉

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