Detection of ESAT-6 by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis

Mohamed Fethi Diouani 1, 2, 3 Oussama Ouerghi 4, 5 Amira Refai 6, 5 Kamel Belgacem 1, 5 Chaker Tlili 1, 7 Dhafer Laouini 6, 5 Makram Essafi 8, 6
1 LR11IPT03 - Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire
2 LR11-IPT-02 - Laboratory of Transmission, Control and Immunobiology of Infection
3 Faculty of Sciences of Bizerte
4 PSAU - Prince Sattam Bin Abdul-Aziz University
5 Tunis El Manar University
6 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections
7 CIGIT - Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences
8 Université Tunis El Manar, Tunis, Tunisia
Abstract : Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein. Anti-ESAT-6 antibodies were first covalently immobilized on the surface of a gold screen-printed electrode functionalized via a self-assembled thiol monolayer. Interaction between the bio-receptor and ESAT-6 antigen was evaluated by square wave voltammetry method using [Fe(CN)6](3-/4-) as redox probe. The detection limit of ESAT-6 antigen was 7ng/ml. The immunosensor has also been able to detect native ESAT-6 antigen secreted in cell culture filtrates of three pathogenic strains of Mtb (CDC1551, H37RV and H8N8). Overall, this work describes an immune-electrochemical biosensor, based on ESAT-6 antigen detection, as a useful diagnostic tool for tuberculosis.
Keywords : Biosensor Diagnosis ESAT-6 Immuno-sensor Square wave voltammetry Tuberculosis
Type de document :
Article dans une revue
Materials Science and Engineering: C, Elsevier, 2017, 74, pp.465 - 470. 〈10.1016/j.msec.2016.12.051〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01592766
Contributeur : Mohamed Fethi Diouani <>
Soumis le : lundi 25 septembre 2017 - 13:15:53
Dernière modification le : lundi 5 février 2018 - 15:22:14

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Mohamed Fethi Diouani, Oussama Ouerghi, Amira Refai, Kamel Belgacem, Chaker Tlili, et al.. Detection of ESAT-6 by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis. Materials Science and Engineering: C, Elsevier, 2017, 74, pp.465 - 470. 〈10.1016/j.msec.2016.12.051〉. 〈pasteur-01592766〉

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