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Detection of ESAT-6 by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis

Abstract : Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein. Anti-ESAT-6 antibodies were first covalently immobilized on the surface of a gold screen-printed electrode functionalized via a self-assembled thiol monolayer. Interaction between the bio-receptor and ESAT-6 antigen was evaluated by square wave voltammetry method using [Fe(CN)6](3-/4-) as redox probe. The detection limit of ESAT-6 antigen was 7ng/ml. The immunosensor has also been able to detect native ESAT-6 antigen secreted in cell culture filtrates of three pathogenic strains of Mtb (CDC1551, H37RV and H8N8). Overall, this work describes an immune-electrochemical biosensor, based on ESAT-6 antigen detection, as a useful diagnostic tool for tuberculosis.
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https://hal-riip.archives-ouvertes.fr/pasteur-01592766
Contributor : Mohamed Fethi Diouani <>
Submitted on : Monday, September 25, 2017 - 1:15:53 PM
Last modification on : Wednesday, October 28, 2020 - 9:52:05 AM

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Mohamed Fethi Diouani, Oussama Ouerghi, Amira Refai, Kamel Belgacem, Chaker Tlili, et al.. Detection of ESAT-6 by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis. Materials Science and Engineering: C, Elsevier, 2017, 74, pp.465 - 470. ⟨10.1016/j.msec.2016.12.051⟩. ⟨pasteur-01592766⟩

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