Development of molecular and cellular tools to decipher the type I IFN pathway of the common vampire bat

Sarkis Sarkis (1) , Marie-Claude Lise (1) , Edith Darcissac (1) , Stéphanie Dabo (2) , Marcel Falk (1) , Laura Chaulet (1) , Christine Neuveut (2) , Eliane Meurs (2) , Anne Lavergne (1) , Vincent Lacoste (1)

1 Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française]
2 Hépacivirus et immunité innée

Though the common vampire bat, Desmodus rotundus, is known as the main rabies virus reservoir in Latin America, no tools are available to investigate its antiviral innate immune system. To characterize the IFN-I pathway, we established an immortalized cell line from a D. rotundus fetal lung named FLuDero. Then we molecularly characterized some of the Toll-like receptors (TLR3, 7, 8 and 9), the three RIG-I-like receptor members, as well as IFNa1 and IFNb. Challenging the FLuDero cell line with poly (I:C) resulted in an up-regulation of both IFNa1 and IFNb and the induction of expression of the different pattern recognition receptors characterized. These findings provide evidence of the intact dsRNA recognition machinery and the IFN-I signaling pathway in our cellular model. Herein, we generated a sum of insightful specific molecular and cellular tools that will serve as a useful model to study virusehost interactions of the common vampire bat.

Mots clés: Type I interferon; Cell line; Desmodus rotundus; Innate immunity; Toll-like receptor; RIG-I-Like receptor

Domaines: Immunité innée; Virologie

Sarkis Sarkis, Marie-Claude Lise, Edith Darcissac, Stéphanie Dabo, Marcel Falk, et al.. Development of molecular and cellular tools to decipher the type I IFN pathway of the common vampire bat. Developmental and Comparative Immunology, 2017, 81, pp.1 - 7. ⟨10.1016/j.dci.2017.10.023⟩. ⟨pasteur-01633796⟩