Assessment of tuberculosis spatial hotspot areas in Antananarivo, Madagascar, by combining spatial analysis and genotyping

Noël Harijaona Ratovonirina (1, 2) , Niaina Rakotosamimanana (1) , Solohery Lalaina Razafimahatratra (1) , Mamy Serge Raherison (1, 3) , Guislaine Refrégier (2) , Christophe Sola (2) , Fanjasoa Rakotomanana (4) , Voahangy Rasolofo Razanamparany (1)

1 IPM - Unité des Mycobactéries [Antananarivo, Madagascar]
2 IGM - Institut de génétique et microbiologie [Orsay]
3 Ministère de la Santé Publique [Antananarivo, Madagascar]
4 Institut Pasteur de Madagascar

Background: Tuberculosis (TB) remains a public health problem in Madagascar. A crucial element of TB control is the development of an easy and rapid method for the orientation of TB control strategies in the country. Our main objective was to develop a TB spatial hotspot identification method by combining spatial analysis and TB genotyping method in Antananarivo. Methods: Sputa of new pulmonary TB cases from 20 TB diagnosis and treatment centers (DTCs) in Antananarivo were collected from August 2013 to May 2014 for culture. Mycobacterium tuberculosis complex (MTBC) clinical isolates were typed by spoligotyping on a Luminex® 200 platform. All TB patients were respectively localized according to their neighborhood residence and the spatial distribution of all pulmonary TB patients and patients with genotypic clustered isolates were scanned respectively by the Kulldorff spatial scanning method for identification of significant spatial clustering. Areas exhibiting spatial clustering of patients with genotypic clustered isolates were considered as hotspot TB areas for transmission. Results: Overall, 467 new cases were included in the study, and 394 spoligotypes were obtained (84.4%). New TB cases were distributed in 133 of the 192 Fokontany (administrative neighborhoods) of Antananarivo (1 to 15 clinical patients per Fokontany) and patients with genotypic clustered isolates were distributed in 127 of the 192 Fokontany (1 to 13 per Fokontany). A single spatial focal point of epidemics was detected when ignoring genotypic data (p = 0.039). One Fokontany of this focal point and three additional ones were detected to be spatially clustered when taking genotypes into account (p < 0.05). These four areas were declared potential TB transmission hotspots in Antananarivo and will be considered as priority targets for surveillance in the future. Conclusion: This method, combining spatial analysis and TB genotyping will now be used for further focused clinical and epidemiological studies in Madagascar and will allow better TB control strategies by public health authorities.

Keywords: Antananarivo; Genotyping; Geographic Information System; Mycobacterium tuberculosis; Spatial cluster

Domains: Life Sciences [q-bio]; Santé publique et épidémiologie; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Noël Harijaona Ratovonirina, Niaina Rakotosamimanana, Solohery Lalaina Razafimahatratra, Mamy Serge Raherison, Guislaine Refrégier, et al.. Assessment of tuberculosis spatial hotspot areas in Antananarivo, Madagascar, by combining spatial analysis and genotyping. BMC Infectious Diseases, 2017, 17 (1), pp.562. ⟨10.1186/s12879-017-2653-9⟩. ⟨pasteur-01649214⟩