Mapping of Mycobacterium tuberculosis Complex Genetic Diversity Profiles in Tanzania and Other African Countries

Erasto V Mbugi (1) , Bugwesa Z. Katale (1, 2) , Elizabeth M. Streicher (3) , Julius D. Keyyu (2) , Sharon L. Kendall (4) , Hazel M. Dockrell (5) , Anita L. Michel (6) , Mark M. Rweyemamu (7) , Robin M. Warren (3) , Mecky I. Matee (1) , Paul D. van Helden (3) , David Couvin (8) , Nalin Rastogi (8)

1 Muhimbili University of Health and Allied Sciences
2 TAWIRI - Tanzania Wildlife Research Institute
3 DST/NRF Centre of Excellence in Strong Materials (Johannesburg, South Africa )
4 LSHTM - London School of Hygiene and Tropical Medicine
5 Royal Veterinary College [London]
6 University of Pretoria [South Africa]
7 SUA - Sokoine University of Agriculture
8 Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory

The aim of this study was to assess and characterize Mycobacterium tuberculosis complex (MTBC) genotypic diversity in Tanzania, as well as in neighbouring East and other several African countries. We used spoligotyping to identify a total of 293 M. tuberculosis clinical isolates (one isolate per patient) collected in the Bunda, Dar es Salaam, Ngorongoro and Serengeti areas in Tanzania. The results were compared with results in the SITVIT2 international database of the Pasteur Institute of Guadeloupe. Genotyping and phylogeographi-cal analyses highlighted the predominance of the CAS, T, EAI, and LAM MTBC lineages in Tanzania. The three most frequent Spoligotype International Types (SITs) were: SIT21/ CAS1-Kili (n = 76; 25.94%), SIT59/LAM11-ZWE (n = 22; 7.51%), and SIT126/EAI5 tentatively reclassified as EAI3-TZA (n = 18; 6.14%). Furthermore, three SITs were newly created in this study (SIT4056/EAI5 n = 2, SIT4057/T1 n = 1, and SIT4058/EAI5 n = 1).

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology; Life Sciences [q-bio]; Santé publique et épidémiologie; Life Sciences [q-bio]; Genetics; Populations and Evolution [q-bio.PE]

Erasto V Mbugi, Bugwesa Z. Katale, Elizabeth M. Streicher, Julius D. Keyyu, Sharon L. Kendall, et al.. Mapping of Mycobacterium tuberculosis Complex Genetic Diversity Profiles in Tanzania and Other African Countries. PLoS ONE, 2016, 11 (5), pp.e0154571. ⟨10.1371/journal.pone.0154571⟩. ⟨pasteur-01652118⟩