Skip to Main content Skip to Navigation
Journal articles

Mapping of Mycobacterium tuberculosis Complex Genetic Diversity Profiles in Tanzania and Other African Countries

Erasto Mbugi 1, * Bugwesa Z. Katale 1, 2 Elizabeth M. Streicher 3 Julius D. Keyyu 2 Sharon L. Kendall 4 Hazel M. Dockrell 5 Anita L. Michel 6 Mark M. Rweyemamu 7 Robin M. Warren 3 Mecky I. Matee 1 Paul D. van Helden 3 David Couvin 8 Nalin Rastogi 8
* Corresponding author
1 Muhimbili University of Health and Allied Sciences
2 TAWIRI - Tanzania Wildlife Research Institute
3 DST/NRF Centre of Excellence in Strong Materials (Johannesburg, South Africa )
4 LSHTM - London School of Hygiene and Tropical Medicine
5 Royal Veterinary College [London]
6 University of Pretoria [South Africa]
7 SUA - Sokoine University of Agriculture
8 Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory
Abstract : The aim of this study was to assess and characterize Mycobacterium tuberculosis complex (MTBC) genotypic diversity in Tanzania, as well as in neighbouring East and other several African countries. We used spoligotyping to identify a total of 293 M. tuberculosis clinical isolates (one isolate per patient) collected in the Bunda, Dar es Salaam, Ngorongoro and Serengeti areas in Tanzania. The results were compared with results in the SITVIT2 international database of the Pasteur Institute of Guadeloupe. Genotyping and phylogeographi-cal analyses highlighted the predominance of the CAS, T, EAI, and LAM MTBC lineages in Tanzania. The three most frequent Spoligotype International Types (SITs) were: SIT21/ CAS1-Kili (n = 76; 25.94%), SIT59/LAM11-ZWE (n = 22; 7.51%), and SIT126/EAI5 tentatively reclassified as EAI3-TZA (n = 18; 6.14%). Furthermore, three SITs were newly created in this study (SIT4056/EAI5 n = 2, SIT4057/T1 n = 1, and SIT4058/EAI5 n = 1).
Document type :
Journal articles
Complete list of metadata

Cited literature [66 references]  Display  Hide  Download
https://hal-riip.archives-ouvertes.fr/pasteur-01652118
Contributor : Nalin Rastogi <>
Submitted on : Wednesday, November 29, 2017 - 10:58:34 PM
Last modification on : Sunday, November 22, 2020 - 12:52:02 PM

File

Vignette du document
PlosOne2016-Tanzania-Suppl.pdf
Publisher files allowed on an open archive

Licence

Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

RIIP | RIIP_GUADELOUPE

Citation

Erasto Mbugi, Bugwesa Z. Katale, Elizabeth M. Streicher, Julius D. Keyyu, Sharon L. Kendall, et al.. Mapping of Mycobacterium tuberculosis Complex Genetic Diversity Profiles in Tanzania and Other African Countries. PLoS ONE, Public Library of Science, 2016, 11 (5), pp.e0154571. ⟨10.1371/journal.pone.0154571⟩. ⟨pasteur-01652118⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

117

Files downloads

181