Ecophysiological characterization and molecular differentiation of Culex pipiens forms (Diptera: Culicidae) in Tunisia

Abstract : Background: The Culex pipiens complex (Diptera: Culicidae) includes the most widespread mosquito species in the world. Members of this complex are the primary enzootic and epidemic vectors of the West Nile virus (genus Flavivirus) in several countries. The two recognized forms of Cx. pipiens (Linnaeus, 1758)-pipiens and molestus-exhibit behavioral and physiological differences. Natural populations of Cx. pipiens were investigated in several sites in Tunisia to evaluate the ecophysiological and molecular characteristics of their forms. Results: The analysis showed the sympatric presence of Cx. pipiens forms and hybrids in all studied sites. Of all the tested larvae of Cx. pipiens, 33.5% were identified as pipiens, 30.8% were identified as molestus, and 35.6% were identified as hybrids. The molestus and hybrid forms were positively correlated with urban habitats and belowground sites while the pipiens form was positively correlated with rural habitats and aboveground sites. Autogeny was expressed in all types of habitats and breeding sites. By contrast with the microsatellite CQ11, the two molecular markers, ace-2 and cytb, did not allow differentiation between the Cx. pipiens forms. Conclusions: Our study shows the ubiquitous distribution and the plasticity of the different forms of Cx. pipiens in a wide range of ecological conditions. It suggests that the behavioral traits assigned to the forms of Cx. pipiens seem to be more flexible than previously assumed. Our analysis also proves that the microsatellite CQ11 remains an efficient tool for distinguishing between Cx. pipiens forms.
Type de document :
Article dans une revue
Parasites and Vectors, BioMed Central, 2017, 10 (1), pp.327. 〈10.1186/s13071-017-2265-7〉
Liste complète des métadonnées

Littérature citée [54 références]  Voir  Masquer  Télécharger

https://hal-riip.archives-ouvertes.fr/pasteur-01660862
Contributeur : Abdelhakim Ben Hassine <>
Soumis le : lundi 11 décembre 2017 - 14:37:32
Dernière modification le : lundi 8 octobre 2018 - 17:44:08

Fichier

Ecophysiological.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Marwa Beji, Adel Rhim, David Roiz, Ali Bouattour. Ecophysiological characterization and molecular differentiation of Culex pipiens forms (Diptera: Culicidae) in Tunisia. Parasites and Vectors, BioMed Central, 2017, 10 (1), pp.327. 〈10.1186/s13071-017-2265-7〉. 〈pasteur-01660862〉

Partager

Métriques

Consultations de la notice

261

Téléchargements de fichiers

50