Reverse Transcriptase Mutations in Cambodian CRF01_AE Isolates after Antiretroviral Prophylaxis against HIV Type 1 Perinatal Transmission

Nary Ly (1) , Viseth Phoung (1) , Duong Chan Min (1) , Chanthan Srey (1) , Leang Sim Kruy (2) , Kanal Koum (2) , Vannarith Chhum (3) , Philippe Glaziou (1) , Hervé Fleury (4) , Jean-Marc Reynes (1)

1 Institut Pasteur du Cambodge
2 Calmette Hospital [Phnom Penh]
3 Provincial Health Department
4 Variabilité génomique des virus

This study explores amino acid changes of the reverse transcriptase (rt) of CRF01_AE isolates from pregnant women naive to antiretroviral drugs before and 2, 6, and 52 weeks after exposure to single dose nevirapine (sdNVP). Results based on 51 observations showed that the proportion of isolates with nonnucleoside reverse transcriptase inhibitor (NNRTI) RMs in the group treated with sdNVP (n = 35) increased from 0% pre-NVP to 22.9% at week 2 postpartum (pp) and 22.9% at week 6 pp. In the group treated with zidovudine + sdNVP (n = 16), the proportion with RM was 31.3% and 18.8% at weeks 2 and 6 pp, respectively. Only a few RMs were still detected at week 52 pp. No apparent subtype-specific treatment-related mutations were detected. NNRTI RM occurrence in CRF01_AE strains is similar to subtype A, D, and CRF02_AG strains after exposure to antiretroviral drugs for PMTCT.

Domains: Infectious diseases; Santé publique et épidémiologie; Virology

Nary Ly, Viseth Phoung, Duong Chan Min, Chanthan Srey, Leang Sim Kruy, et al.. Reverse Transcriptase Mutations in Cambodian CRF01_AE Isolates after Antiretroviral Prophylaxis against HIV Type 1 Perinatal Transmission. AIDS Research and Human Retroviruses, 2007, 23 (12), pp.1563 - 1568. ⟨10.1089/aid.2007.0100⟩. ⟨pasteur-01835655⟩