Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs).

François Lépine (1) , Sylvain Milot (1) , Marie-Christine Groleau (1) , Eric Déziel (1)

1 INRS-IAF - Institut Armand Frappier

High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.

Keywords: 4-Hydroxy-2-alkylquinoline; Acyl-homoserine lactone; Analysis; Bacteria; Liquid chromatography; Mass spectrometry; Quantification; Quorum sensing

Domains: Life Sciences [q-bio]

François Lépine, Sylvain Milot, Marie-Christine Groleau, Eric Déziel. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs).. Quorum Sensing, Methods and Protocols, 1673, Humana Press, pp.49-59, 2018, Methods in Molecular Biology, 978-1-4939-7309-5. ⟨10.1007/978-1-4939-7309-5_4⟩. ⟨pasteur-01854548⟩