The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology

Jenny Stodola 1 Guillaume Dubois 1 Alain Le Coupanec 1 Marc Desforges 1, * Pierre Talbot 1, *
* Corresponding author
1 INRS-IAF - Institut Armand Frappier
Abstract : The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various roles including in virion morphology/assembly and in cell response to infection and/or virulence. Making use of recombinant viruses either devoid of the E protein or harboring mutations either in putative transmembrane domain or PDZ-binding motif, we demonstrated that a fully functional HCoV-OC43 E protein is first needed for optimal production of recombinant infectious viruses. Furthermore, HCoV-OC43 infection of human epithelial and neuronal cell lines, of mixed murine primary cultures from the central nervous system and of mouse central nervous system showed that the E protein is critical for efficient and optimal virus replication and propagation, and thereby for neurovirulence.
Keywords : Transmembrane domain Virus production Pathogenesis Coronavirus E protein HCoV-OC43 PDZ binding motif
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Journal articles
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Submitted on : Monday, August 6, 2018 - 8:16:33 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM

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Jenny Stodola, Guillaume Dubois, Alain Le Coupanec, Marc Desforges, Pierre Talbot. The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology. Virology, Elsevier, 2018, 515, pp.134 - 149. ⟨10.1016/j.virol.2017.12.023⟩. ⟨pasteur-01854621⟩

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