T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro - Archive ouverte HAL Access content directly
Journal Articles Marine Pollution Bulletin Year : 2018

T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro

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Christine Kleinert
  • Function : Correspondent author
  • PersonId : 1035897

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Emilie Lacaze
  • Function : Author
  • PersonId : 764460
  • IdRef : 152621873
Mike O. Hammill
  • Function : Author
  • PersonId : 885360

Abstract

The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals.
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Dates and versions

pasteur-01855960 , version 1 (08-08-2018)

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Christine Kleinert, Emilie Lacaze, Marlène Fortier, Mike O. Hammill, Sylvain de Guise, et al.. T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro. Marine Pollution Bulletin, 2018, 127, pp.225 - 234. ⟨10.1016/j.marpolbul.2017.12.001⟩. ⟨pasteur-01855960⟩

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