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T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro

Abstract : The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals.
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https://hal-riip.archives-ouvertes.fr/pasteur-01855960
Contributor : Michel Courcelles <>
Submitted on : Wednesday, August 8, 2018 - 10:03:33 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM

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Christine Kleinert, Emilie Lacaze, Marlène Fortier, Mike Hammill, Sylvain de Guise, et al.. T lymphocyte-proliferative responses of harbor seal ( Phoca vitulina ) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro. Marine Pollution Bulletin, Elsevier, 2018, 127, pp.225 - 234. ⟨10.1016/j.marpolbul.2017.12.001⟩. ⟨pasteur-01855960⟩

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