Anti-angiogenic effect of phospholipases A 2 from Scorpio maurus venom glands on Human Umbilical Vein Endothelial Cells

Abstract : In a previous study, we purified Sm-PLGV an heterodimeric phospholipase A2, from the venom glands of the Tunisian scorpion Scorpio maurus. This enzyme contains a Long chain, a penta-peptide insertion, which is cut out during the maturation process, followed by a short chain. A disulfide bridge links the two chains. Three recombinant forms of this enzyme were produced in Escherichia coli: rPLA2(+5) with a penta-peptide insert, rPLA2(-5) without the penta-peptide, and the Long chain alone without the short one. In the present study, we showed that Sm-PLGV, rPLA2(+5) and rPLA2(-5) displayed more potent anti-angiogenic activity in vitro than the recombinant Long chain and the short one obtained by chemical synthesis. These phospholipases A2 inhibited in a dose-dependent manner adhesion, migration and invasion of Human Umbilical Vein Endothelial Cells. Using Matrigel™, we demonstrated that Sm-PLGV, rPLA2(+5) and rPLA2(-5) significantly inhibited tubulogensesis. We also showed a clear dissociation between the anti-angiogenic effect of Sm-PLGV and its catalytic activity.
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Submitted on : Friday, December 14, 2018 - 9:41:06 AM
Last modification on : Tuesday, May 21, 2019 - 7:19:14 PM

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Najeh Krayem, Zaineb Abdelkefi-Koubaa, Naziha Marrakchi, José Luis, Youssef Gargouri. Anti-angiogenic effect of phospholipases A 2 from Scorpio maurus venom glands on Human Umbilical Vein Endothelial Cells. Toxicon, Elsevier, 2018, 145, pp.6-14. ⟨10.1016/j.toxicon.2018.02.042⟩. ⟨pasteur-01954950⟩

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