Quelle technique adopter pour le phénotypage des alvéolites lymphocytaires : immunocytochimie ou cytométrie en flux ?

Abstract : Background : Diffuse interstitial pneumonias are considered as a group of multiple affections characterized by challenging diagnoses because of the lack of specific clinical signs. Radiologic investigations highlight the diagnoses in most of the cases but bronchoalveolar lavage plays a key role in the diagnostic diagram. We aim to compare the immunocytochemical technique and the flow cytometry in the phenotyping of lymphocytic alveolitis. Methods : We described a series of 32 lymphocytic alveolitis, which were analyzed using immunocytochemistry and flow cytometry. Results : We found a good reproducibility between the immunocytochemistry performed on smears and cytoblocks (kappa = 0.7) and a poor reproducibility between immunocytochemistry and flow cytometry (kappa = 0.35). Conclusion : Our study emphasized on the poor reproducibility between immunocytochemistry and flow cytometry. Further studies about the reliability of both techniques are needed especially in discordant cases.
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Submitted on : Wednesday, December 19, 2018 - 9:54:52 AM
Last modification on : Friday, July 26, 2019 - 1:24:04 PM

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Mona Mlika, Rihem Kasmi, Ines Safra, Emna Braham, Chokri Chebbi, et al.. Quelle technique adopter pour le phénotypage des alvéolites lymphocytaires : immunocytochimie ou cytométrie en flux ?. Annales de Pathologie, Elsevier Masson, 2017, 37 (5), pp.347-354. ⟨10.1016/j.annpat.2017.07.002⟩. ⟨pasteur-01959942⟩

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