Specific immune responses in mice following subchronic exposure to acetamiprid

Soumaya Marzouki (1) , Ines Bini Dhouib (2) , Chaouki Benabdessalem (1) , Raja Rekik (1) , Raoudha Doghri (3) , Ammar Maroueni (4) , Zakaria Bellasfar (4) , Saloua Fazaa (5) , Jihène Bettaieb (1, 6) , Mohamed Ridha Barbouche (1, 6) , Mélika Ben Ahmed (1, 6)

1 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection
2 LR11IPT08 - Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules
3 Institut Salah Azaiez de Cancer
4 Institut Pasteur de Tunis
5 FST - Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis
6 Faculté de Médecine de Tunis

AIMS: Acetamiprid (ACE) is an insecticide of the neonicotinoid family, the most widely used in the world. Herein, we assessed the effect of ACE on either the humoral or cellular immune responses of rodents. We also evaluated the role of curcumin in the restoration of altered immune responses after ACE treatment. METHODS: Five groups of five Swiss Albino mice were immunized intraperitoneally with the recombinant form of CFP32, a virulence factor of Mycobacterium tuberculosis. One group received ACE (5mg/kg) during 61days, a second one received ACE associated with curcumin (100mg/kg). Three control groups were included; one untreated, the second received corn oil and the third received curcumin alone. The humoral immune response was assessed by ELISA testing the anti-rCFP32 antibody concentrations in the serum. The cellular immune response was assessed by analyzing the cellular proliferation of the splenocytes stimulated in vitro by a mitogen or rCFP32. RESULTS: The ACE-treated mice showed a significant immunosuppression of the specific humoral response with a restorative effect of curcumin when administered with ACE. Similarly, ACE significantly decreased the level of splenocyte proliferation after either a non specific or a specific activation. Curcumin partially restores the antigen specific cellular immune response. Moreover, when administered alone, curcumin significantly inhibits the proliferative responses to the mitogen confirming its anti-mitogenic effect. Histological analysis showed alteration of spleens of mice exposed to ACE. SIGNIFICANCE: Altogether, our data indicated that ACE could potentially be harmful to the immune system.

Mots clés: Acetamiprid; Curcumin; Immunotoxicity; Specific immune response; Subchronic exposure

Domaines: Sciences du Vivant [q-bio]; Immunologie

Soumaya Marzouki, Ines Bini Dhouib, Chaouki Benabdessalem, Raja Rekik, Raoudha Doghri, et al.. Specific immune responses in mice following subchronic exposure to acetamiprid. Life Sciences, 2018, 188, pp.10-16. ⟨10.1016/j.lfs.2017.08.022⟩. ⟨pasteur-01959976⟩