Targetting alphavbeta3 and alpha5beta1 integrins with Ecballium elaterium (L.) A. Rich. seed oil

Imen Touihri-Barakati (1) , Olfa Kallech-Ziri (1) , Abdennacer Boulila (1) , Khaoula Khwaldia (1) , Naziha Marrakchi (2, 3) , Belgacem Hanchi (4) , Karim Hosni (1) , José Luis (5)

1 LR02INRAP10 - Laboratoire des Substances Naturelles
2 LR11IPT08 - Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules
3 Faculté de Médecine de Tunis
4 FST - Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis
5 CRO2 - Centre de Recherches en Oncologie biologique et Oncopharmacologie

In the present study, the effect of Ecbalium elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel TM assays, the oil significantly inhibited angiogenesis. The anti-tumor effect of the oil is specifically mediated by avb3 and a5b1 integrins. The presence of integrin antagonists in seed oil from E. elaterium could be used for the development of anticancer drugs with targeted " multi-modal " therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.

Keywords: Seed oil; αvβ3 and α5β1 integrins; Ecballium elaterium; Glioma cell line; Angiogenesis; Adhesion

Domains: Cancer; Life Sciences [q-bio]

Imen Touihri-Barakati, Olfa Kallech-Ziri, Abdennacer Boulila, Khaoula Khwaldia, Naziha Marrakchi, et al.. Targetting alphavbeta3 and alpha5beta1 integrins with Ecballium elaterium (L.) A. Rich. seed oil. Biomedicine and Pharmacotherapy, 2016, 84, pp.1223-1232. ⟨10.1016/j.biopha.2016.10.035⟩. ⟨hal-01474971⟩