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Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA

Abstract : This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
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https://hal-riip.archives-ouvertes.fr/pasteur-01973057
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Submitted on : Tuesday, January 8, 2019 - 10:14:58 AM
Last modification on : Friday, February 18, 2022 - 4:36:01 PM

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Mahamoudou Ouattara, Melissa Whaley, Laurel T Jenkins, Stephanie Schwartz, Rasmata Ouédraogo Traoré, et al.. Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA. Diagnostic Microbiology and Infectious Disease, Elsevier, 2018, 93 (3), pp.188-190. ⟨10.1016/j.diagmicrobio.2018.10.008⟩. ⟨pasteur-01973057⟩

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