Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA

Mahamoudou Ouattara 1 Melissa Whaley 1 Laurel Jenkins 1 Stephanie Schwartz 1 Rasmata Traoré 1 Seydou Diarra 2 Jean-Marc Collard 3, 4 Claudio Sacchi 5 Xin Wang 1
1 CDC - Centers for Disease Control and Prevention [Atlanta]
2 INRSP - Institut National de Recherche en Santé Publique [Bamako]
3 Institut Pasteur de Madagascar
4 CERMES - Centre de Recherche Médicale et Sanitaire (Niamey, Niger)
5 Instituto Adolfo Lutz [São Paulo, Brazil]
Abstract : This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
Keywords : Cerebrospinal Bacteria Triplex Detection Meningitis Real-time PCR
Document type :
Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-01973057
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Submitted on : Tuesday, January 8, 2019 - 10:14:58 AM
Last modification on : Saturday, July 20, 2019 - 1:02:11 AM

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Mahamoudou Ouattara, Melissa Whaley, Laurel Jenkins, Stephanie Schwartz, Rasmata Traoré, et al.. Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA. Diagnostic Microbiology and Infectious Disease, Elsevier, 2018, 93 (3), pp.188-190. ⟨10.1016/j.diagmicrobio.2018.10.008⟩. ⟨pasteur-01973057⟩

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