A model to study the effects of a viral inactivator (beta-propiolactone) on DNA ligation and gene expression in E. coli and Cos cells. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Vaccine Année : 1999

A model to study the effects of a viral inactivator (beta-propiolactone) on DNA ligation and gene expression in E. coli and Cos cells.

Résumé

An experimental model to study the effects of viral inactivators on the biological properties of DNA was developed. Beta-propiolactone (betaPL) was used in this model and its effects on ligation, transfer and gene expression of naked DNA were assessed. Evidence that betaPL impairs these two major DNA functions are presented. The amounts of betaPL that alter or abolish gene expression and prevent DNA cohesive ends ligation were determined. Based on these observations, it was concluded that this experimental approach could be used to study the effects on the biological properties of DNA of other inactivators used in vaccine preparations.

Dates et versions

pasteur-01985535 , version 1 (18-01-2019)

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Dahmani M. Fathallah, K. Zerria, M Barbouche, K. Dellagi. A model to study the effects of a viral inactivator (beta-propiolactone) on DNA ligation and gene expression in E. coli and Cos cells.. Vaccine, 1999, 17 (1), pp.95-98. ⟨10.1016/S0264-410X(98)00087-5⟩. ⟨pasteur-01985535⟩

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