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Generating genomic platforms to study Candida albicans pathogenesis

Abstract : The advent of the genomic era has made elucidating gene function on a large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources toward this endeavor. Here we provide novel, genome-scale tools for the study of Candida albicans, a commen-sal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http: //candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags and selection markers and demonstrated their applicability to the study of target ORFs transferred from the C. albicans ORFeome. In addition, the use of the OR-Feome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were en
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Mélanie Legrand, Sophie Bachellier-Bassi, Keunsook Lee, Yogesh Chaudhari, Hélène Tournu, et al.. Generating genomic platforms to study Candida albicans pathogenesis. Nucleic Acids Research, Oxford University Press, 2018, 46 (14), pp.6935-6949. ⟨10.1093/nar/gky594⟩. ⟨pasteur-02015988⟩

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