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Journal articles
Generating genomic platforms to study Candida albicans pathogenesis
Mélanie Legrand
1
Sophie Bachellier-Bassi
1
Keunsook Lee
2
Yogesh Chaudhari
2
Hélène Tournu
3
Laurence Arbogast
1
Hélène Boyer
1
Murielle Chauvel
1
Vitor Cabral
1
Corinne Maufrais
1, 4
Audrey Nesseir
1
Irena Maslanka
2
Emmanuelle Permal
1
Tristan Rossignol
1
Louise Walker
2
Ute Zeidler
1
Sadri Znaidi
1
Floris Schoeters
3
Charlotte Majgier
1
Renaud Julien
5
Laurence Ma
6
Magali Tichit
6
Christiane Bouchier
6
Patrick Van dijck
3
Carol Munro
2
Christophe D’enfert
1
Mélanie Legrand 1
Author
Sophie Bachellier-Bassi 1
Author
Keunsook A Lee 2
Author
Yogesh Chaudhari 2
Author
Vitor Cabral 1
Author
Corinne Maufrais 1, 4
Author IdHAL : corinne-maufrais ORCID : https://orcid.org/0000-0002-8718-0432
Audrey Nesseir 1
Author
Irena Maslanka 2
Author
Emmanuelle E. Permal 1
Author
Tristan Rossignol 1
Author IdHAL : tristan-rossignol ResearcherId : http://www.researcherid.com/rid/B-9398-2008 ORCID : https://orcid.org/0000-0003-0718-0684
Louise A Walker 2
Author
Ute Zeidler 1
Author
Carol A Munro 2
Author
Christophe D’enfert 1
Author
1
BPF - Biologie et Pathogénicité fongiques (Département de Mycologie - 25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INRA - Institut National de la Recherche Agronomique : USC2019 (France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
2
University of Aberdeen (King's College, Aberdeen AB24 3FX - United Kingdom)
3
KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven (Oude Markt 13 - bus 5005, 3000 Leuven - Belgium)
4
Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB (Département de biologie computationnelle (ex C3BI), 25-28 rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : USR3756 (France)
6
Biomics (25-28 rue du Docteur Roux 75724 Paris Cedex 15 - France)
- CITECH - Centre de Recherche et Innovation Technologique (25-28, rue du Docteur Roux, 75724 Paris cedex 15 - )
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
Abstract : The advent of the genomic era has made elucidating gene function on a large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources toward this endeavor. Here we provide novel, genome-scale tools for the study of Candida albicans, a commen-sal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http: //candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags and selection markers and demonstrated their applicability to the study of target ORFs transferred from the C. albicans ORFeome. In addition, the use of the OR-Feome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were en
Document type :
Journal articles
Complete list of metadata
https://hal-riip.archives-ouvertes.fr/pasteur-02015988
Contributor : Abdelhakim Ben Hassine Connect in order to contact the contributor
Submitted on : Tuesday, February 12, 2019 - 3:09:48 PM
Last modification on : Monday, January 17, 2022 - 1:58:03 PM
Long-term archiving on: : Monday, May 13, 2019 - 4:04:59 PM
Contributor : Abdelhakim Ben Hassine Connect in order to contact the contributor
Submitted on : Tuesday, February 12, 2019 - 3:09:48 PM
Last modification on : Monday, January 17, 2022 - 1:58:03 PM
Long-term archiving on: : Monday, May 13, 2019 - 4:04:59 PM
Licence
Distributed under a Creative Commons Attribution 4.0 International License
Identifiers
- HAL Id : pasteur-02015988, version 1
- PUBMED : 29982705
- DOI : 10.1093/nar/gky594
- PRODINRA : 453258
- WOS : 000444131400007
Citation
Mélanie Legrand, Sophie Bachellier-Bassi, Keunsook Lee, Yogesh Chaudhari, Hélène Tournu, et al.. Generating genomic platforms to study Candida albicans pathogenesis. Nucleic Acids Research, Oxford University Press, 2018, 46 (14), pp.6935-6949. ⟨10.1093/nar/gky594⟩. ⟨pasteur-02015988⟩
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