Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Cerastocytin, a new thrombin-like platelet activator from the venom of the Tunisian viper Cerastes cerastes

Abstract : Cerastocytin, a thrombin-like enzyme from the venom of the desert viper, Cerastes cerastes, has been purified to homogeneity by fast performance liquid chromatography (FPLC) on Mono-Q and Mono-S columns. It is a basic protein (isoelectric point higher than 9) made of a single polypeptide chain of 38 kDa. Its N-terminal polypeptide sequence shows strong similarities with other thrombin-like enzymes from snake venoms. Nanomolar concentrations of cerastocytin induce aggregation of blood platelets. This activity is inhibited by chlorpromazine, theophylline and mepacrine, as in the case of platelet aggregation stimulated by low doses of thrombin. Cerastocytin also possesses an amidolytic activity measured with the thrombin chromogenic substrate S-2238. The platelet aggregating activity and the amidolytic activity of cerastocytin were inhibited by PMSF, TPCK, TLCK and soybean trypsin inhibitors, suggesting that cerastocytin is a serine proteinase. On the other hand, both amidolytic activity and platelet aggregating activity of cerastocytin were unaffected by hirudin or by antithrombin III in the presence of heparin. High concentrations of cerastocytin (1–10 μM) also cleaved prothrombin and Factor X.
Complete list of metadata
Contributor : AbdelHakim Ben Hassine Connect in order to contact the contributor
Submitted on : Tuesday, February 19, 2019 - 11:25:08 AM
Last modification on : Thursday, April 7, 2022 - 10:10:25 AM

Links full text




Naziha Marrakchi, Russolina B. Zingali, Habib Karoui, Cassian Bon, Mohamed El Ayeb. Cerastocytin, a new thrombin-like platelet activator from the venom of the Tunisian viper Cerastes cerastes. Biochimica et Biophysica Acta (BBA) - General Subjects, Elsevier, 1995, 1244 (1), pp.147-156. ⟨10.1016/0304-4165(94)00216-k⟩. ⟨pasteur-02024579⟩



Record views