Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes

Abstract : Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn2+ (10−7 to 10−3 M) to mitogen-stimulated T and B cells enhanced [3H]thymidine incorporation. Addition of 10−5 M Zn2+ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10−7 to 10−3 M) to mitogen-stimulated lymphocytes enhanced [3H]thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10−1 M Mn2+ to lymphocyte cultures exerted an inhibitor effect on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10−1 M Mn2+ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn2+ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10−1 M Mn2+ could be reversed by adding 2 mM CaCl2 to the culture.
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Z. Ghanmi, M. Rouabhia, O. Othmane, P.A. Deschaux. Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes. Ecotoxicology and Environmental Safety, Elsevier, 1989, 17 (2), pp.183-189. ⟨10.1016/0147-6513(89)90037-7⟩. ⟨pasteur-02043718⟩

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