Human cutaneous leishmaniasis caused by Leishmania donovani s.l. in Kenya - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Transactions of The Royal Society of Tropical Medicine and Hygiene Année : 1993

Human cutaneous leishmaniasis caused by Leishmania donovani s.l. in Kenya

Résumé

Our laboratory is characterizing Leishmania stabilates and isolates from active leishmaniasis cases. Smears and cultures from aspirates made on different dates from a single lesion on the bridge of the nose of an 18 years old Kenyan male from Nyandarua District contained Leishmania. The isolates, NLB-271 and NLB-271-IA, were characterized by cellulose acetate electrophoresis (CAE) using 20 enzyme systems and by Southern analysis using 2 deoxyribonucleic acid (DNA) probes (pDK10 and pDK20) from a Dakar strain of L. major (MHOM/SN/00/DK1) and a third probe, p7-059 from L. infantum strain ITMAP-263. Digestion of the two Leishmania DNAs with endonucleases HindIII and PstI, followed by hybridization with the 3 probes, revealed DNA fragment banding patterns indistinguishable from those of the L. donovani species complex. The CAE isoenzyme profiles of these 2 Kenyan isolates were indistinguishable from those of Kenyan L. donovani strains we designated as zymodeme Z6. Excluding post-kala-azar dermal leishmaniasis, this constitutes the first human case of cutaneous leishmaniasis caused by L. donovani s.l. in Kenya. Previously, cutaneous leishmaniasis cases in Kenya have been due to L. aethiopica, L. major and L. tropica only.

Domaines

Parasitologie

Dates et versions

pasteur-02044210 , version 1 (21-02-2019)

Identifiants

Citer

Yemane B. Mebrahtu, Guillaume Van Eys, Ikram Guizani, Phillip G. Lawyer, Hannington Pamba, et al.. Human cutaneous leishmaniasis caused by Leishmania donovani s.l. in Kenya. Transactions of The Royal Society of Tropical Medicine and Hygiene, 1993, 87 (5), pp.598-601. ⟨10.1016/0035-9203(93)90101-u⟩. ⟨pasteur-02044210⟩

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