Further characterization and thrombolytic activity in a rat model of a fibrinogenase from Vipera lebetina venom - Archive ouverte HAL Access content directly
Journal Articles Thrombosis Research Year : 1997

Further characterization and thrombolytic activity in a rat model of a fibrinogenase from Vipera lebetina venom

(1) , (2) , (3) , (1) , (1) , (3)
1
2
3

Abstract

Vipera lebetina fibrinogenase (VIF) was shown to render fibrinogen incoagulable and to solubilize fibrin. The fibrinogenolytic activity of this enzyme was found to be 33 mg fibrinogen/min/mg protein. The study of the specificity of this enzyme revealed that it has no effect on purified factor X, prothrombin and protein C and on the specific chromogenic substrates of their active form. Plasminogen was not activated by VlF but slightly degraded. We have also compared the effect of VlF and plasmin on fibrinogen and shown that these two enzymes have a different sites of cleavage. This enzyme inhibited human platelet aggregation on PRP initiated by ADP and collagen but was without effect on the aggregation of washed rabbit platelets using thrombin as agonist. Administration of VlF in rat did not show any necrosis or hemorrhage in treated rats organ's. We therefore, examined the thrombolytic activity of VlF in a rat model of venous thrombosis. Thrombus was produced in the posterior vena cava by injection of human fibrinogen and thrombin. Injection of 5 mg/Kg body weight showed an evident flow restoration after one hour and measurement of the fibrinogen level a decrease of about 30% after 3 hrs. VlF's action is not dependent on plasminogen activators and may act synergistically with them, thereby providing an intriguing potential clinical application for dissolution of blood clots.

Dates and versions

pasteur-02044536 , version 1 (21-02-2019)

Identifiers

Cite

Ammar Gasmi, Ahmed Chabchoub, Sami Guermazi, Habib Karoui, Mohamed Elayeb, et al.. Further characterization and thrombolytic activity in a rat model of a fibrinogenase from Vipera lebetina venom. Thrombosis Research, 1997, 86 (3), pp.233-242. ⟨10.1016/S0049-3848(97)00066-2⟩. ⟨pasteur-02044536⟩

Collections

RIIP RIIP_TUNIS
43 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More