Lebecetin, a C-Lectin Protein from the Venom of<i> Macrovipera lebetina</i> That Inhibits Platelet Aggregation and Adhesion of Cancerous Cells

Sameh Sarray; Najet Srairi; José Luis; Jacques Marvaldi; Mohamed El Ayeb; Naziha Marrakchi

Lebecetin, a C-Lectin Protein from the Venom of Macrovipera lebetina That Inhibits Platelet Aggregation and Adhesion of Cancerous Cells

Sameh Sarray ^{1, *} Najet Srairi ¹ José Luis ² Jacques Marvaldi ² Mohamed El Ayeb ¹ Naziha Marrakchi ^{1, 3}

* Corresponding author

1 Laboratoire des Venins et Toxines, Institut Pasteur de Tunis

2 ISPDCT - Interaction entre Systèmes Protéiques et Différenciation dans la Cellule Tumorale

3 Faculté de Médecine de Tunis

Abstract : A novel C-lectin protein, lebecetin, was purified and characterized from the venom of Macrovipera lebetina. It is a disulfide-linked heterodimer of 15 and 16 kD. The subunits are homologous to each other and to the other snake venom proteins of the C-type (Ca(2+)-dependent) lectin superfamily. Lebecetin shows a potent inhibitory effect on whole blood and washed platelets induced by different agonists. It inhibits the agglutination of human fixed platelets in the presence of ristocetin. Lebecetin also interferes with the adhesion of IGR39 melanoma and HT29D4 adenocarcinoma cells. These two lines adhere to lebecetin used as matrix. Lebecetin is also able to strongly reduce IGR39 and HT29D4 cell adhesion to fibrinogen and laminin, but not to fibronectin and collagen types I and IV, respectively. Adhesion properties of lebecetin may thus involve integrin receptors.

Keywords : Anti-agglutination protein Anti-platelet aggregation Snake venom C-letin protein

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-riip.archives-ouvertes.fr/pasteur-02049503
Contributor : Abdelhakim Ben Hassine <>
Submitted on : Tuesday, February 26, 2019 - 2:34:44 PM
Last modification on : Friday, June 7, 2019 - 1:28:34 AM

Lebecetin, a C-Lectin Protein from the Venom of Macrovipera lebetina That Inhibits Platelet Aggregation and Adhesion of Cancerous Cells

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Lebecetin, a C-Lectin Protein from the Venom of Macrovipera lebetina That Inhibits Platelet Aggregation and Adhesion of Cancerous Cells

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