Antibacterial properties of the pituitary adenylate cyclase-activating polypeptide: A new human antimicrobial peptide

Abstract : The Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), a polycationic, amphiphilic and helical neuropeptide, is well known for its neuroprotective actions and cell penetrating properties. In the present study, we evaluated the potent antibacterial property of PACAP38 and related analogs against various bacterial strains. Interestingly, PACAP38 and related analogs can inhibit the growth of various bacteria including Escherichia coli (JM109), Bacillus subtilis (PY79), and the pathogenic Burkholderia cenocepacia (J2315). Investigation of the mechanism of action suggested that a PACAP metabolite, identified as PACAP(9-38), might indeed be responsible for the observed PACAP38 antibacterial action. Surprisingly, PACAP(9-38), which does not induce haemolysis, exhibits an increased specificity toward Burkholderia cenocepacia J2315 compared to other tested bacteria. Finally, the predisposition of PACAP(9-38) to adopt a π-helix conformation rather than an α-helical conformation like PACAP38 could explain this gain in specificity. Overall, this study has revealed a new function for PACAP38 and related derivatives that can be added to its pleiotropic biological activities. This innovative study could therefore pave the way toward the development of new therapeutic agents against multiresistant bacteria, and more specifically the Burkholderia cenocepacia complex.
Document type :
Journal articles
Complete list of metadatas

Cited literature [33 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-02133267
Contributor : Michel Courcelles <>
Submitted on : Friday, May 17, 2019 - 9:52:01 PM
Last modification on : Thursday, July 11, 2019 - 2:10:09 PM

File

Antibacterial properties of th...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Somia Debbabi, Marie-Christine Groleau, Myriam Létourneau, Chitra Narayanan, Laura-Lee Gosselin, et al.. Antibacterial properties of the pituitary adenylate cyclase-activating polypeptide: A new human antimicrobial peptide. PLoS ONE, Public Library of Science, 2018, 13 (11), ⟨10.1371/journal.pone.0207366⟩. ⟨pasteur-02133267⟩

Share

Metrics

Record views

16

Files downloads

23