Skip to Main content Skip to Navigation
Journal articles

Effect of β-lactam antibiotic resistance gene expression on the radio-resistance profile of E. coli O157:H7

Abstract : Some pathogens might develop favorable global adaptation in response to certain stress treatments resulting in enhanced virulence and/or resistance to a different stress. β-lactam resistance, as well as ampC and ampG genes involved in this resistance, were studied to evaluate their possible role in Escherichia coli O157:H7 (E. coli) radioresistance. E. coli adapted to 25, 15 or 7 μg/mL of kanamycin or carbenicillin, were produced and treated with sensitization (0.4 kGy) or lethal (1.5 kGy) irradiation doses. In E. coli O157:H7, irradiation treatment at 0.4 kGy dose increased ampC and ampG expression respectively by 1.6 and 2-fold in the wild type strain (Wt) but up to by 2.4 and 3.4-fold when the strain was beforehand adapted to 25 μg/mL of carbenicillin (Carb25). Accordingly, ΔampC and ΔampG mutants and E. coli adapted to 25 μg/mL of kanamycin were more sensitive to 0.4 kGy treatment than Wt. While, E. coli Carb25 or overexpression of ampC and ampG provided complete resistance to 0.4 kGy and were even able to survive and grow after exposure to a normally lethal 1.5 kGy irradiation dose. We further noticed that these strains can tolerate other stresses like oxidative, cold and heat shocks. This demonstrates that carbenicillin adaptation promotes resistance to γ-irradiation and to other stresses, likely at least through increased AmpC and AmpG expression. These results are important for the food industry and particularly when considering the use of irradiation for food preservation of meat obtained directly from animals fed β-lactam antibiotics.
Document type :
Journal articles
Complete list of metadatas

Cited literature [49 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-02135715
Contributor : Michel Courcelles <>
Submitted on : Tuesday, May 21, 2019 - 3:15:14 PM
Last modification on : Tuesday, September 3, 2019 - 5:26:06 PM

File

Effect_of_BLactam_antibiotic_r...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Ghizlane Gaougaou, Yosra Ben-Fadhel, Eric Déziel, Lacroix Monique. Effect of β-lactam antibiotic resistance gene expression on the radio-resistance profile of E. coli O157:H7. Heliyon, Elsevier 2018, 4 (12), pp.e00999. ⟨10.1016/j.heliyon.2018.e00999⟩. ⟨pasteur-02135715⟩

Share

Metrics

Record views

42

Files downloads

100