Skip to Main content Skip to Navigation
Journal articles

Detection of human papillomavirus types 45 and 51 by type-specific polymerase chain reaction

Christine Weyn 1 Selma Boulenouar 1 Vanessa Mathys 2 Julie Vanhoolandt 1 Aurore Bernis 1 Véronique Fontaine 1, * Vincent Lacoste 3 Riip And Inctr Workshop Study Group
* Corresponding author
1 Molecular Virology [Brussels]
2 Molecular Pathology of Tuberculosis [Brussels]
3 Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française]
Abstract : Human papillomavirus (HPV) types 45 and 51 are both considered as high risk types for the development of human cervical cancer. To optimize the detection of these two types in clinical samples, HPV-45 and HPV-51 specific primers were designed to amplify respectively a 141bp and a 266bp fragment from the L1 gene by polymerase chain reaction (PCR). The sensitivity and the specificity of these two PCR reactions were determined using varying amounts of HPV DNA containing plasmids and negative and positive controls. Overall, the sensitivity for the HPV-45 plasmid DNA is 10fg, while for HPV-51 the sensitivity is 1fg. This is equivalent to approximately 100 and 10 HPV genome copies per PCR reaction, respectively.
Keywords : HPV-45 HPV-51 Papillomavirus Type-specific PCR Diagnosis
Document type :
Journal articles
Complete list of metadata
https://hal-riip.archives-ouvertes.fr/pasteur-02197626
Contributor : Vincent Lacoste Connect in order to contact the contributor
Submitted on : Tuesday, July 30, 2019 - 3:09:39 PM
Last modification on : Friday, October 23, 2020 - 5:34:02 PM

Identifiers

Collections

RIIP | RIIP_GUYANE

Citation

Christine Weyn, Selma Boulenouar, Vanessa Mathys, Julie Vanhoolandt, Aurore Bernis, et al.. Detection of human papillomavirus types 45 and 51 by type-specific polymerase chain reaction. Journal of Virological Methods, Elsevier, 2007, 146 (1-2), pp.405-408. ⟨10.1016/j.jviromet.2007.08.003⟩. ⟨pasteur-02197626⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

59