Detection of human papillomavirus types 45 and 51 by type-specific polymerase chain reaction

Christine Weyn (1) , Selma Boulenouar (1) , Vanessa Mathys (2) , Julie Vanhoolandt (1) , Aurore Bernis (1) , Véronique Fontaine (1) , Vincent Lacoste (3) , Riip And Inctr Workshop Study Group

1 Molecular Virology [Brussels]
2 Molecular Pathology of Tuberculosis [Brussels]
3 Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française]

Human papillomavirus (HPV) types 45 and 51 are both considered as high risk types for the development of human cervical cancer. To optimize the detection of these two types in clinical samples, HPV-45 and HPV-51 specific primers were designed to amplify respectively a 141bp and a 266bp fragment from the L1 gene by polymerase chain reaction (PCR). The sensitivity and the specificity of these two PCR reactions were determined using varying amounts of HPV DNA containing plasmids and negative and positive controls. Overall, the sensitivity for the HPV-45 plasmid DNA is 10fg, while for HPV-51 the sensitivity is 1fg. This is equivalent to approximately 100 and 10 HPV genome copies per PCR reaction, respectively.

Keywords: HPV-45; HPV-51; Papillomavirus; Type-specific PCR; Diagnosis

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology; Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Christine Weyn, Selma Boulenouar, Vanessa Mathys, Julie Vanhoolandt, Aurore Bernis, et al.. Detection of human papillomavirus types 45 and 51 by type-specific polymerase chain reaction. Journal of Virological Methods, 2007, 146 (1-2), pp.405-408. ⟨10.1016/j.jviromet.2007.08.003⟩. ⟨pasteur-02197626⟩