Enzyme catalysis under pressure

Nicolas Doucet (1)

1 INRS-IAF - Institut Armand Frappier

A strategy using pressure was devised to structurally identify conformational transitions in protein ensembles, allowing the rational prediction of mutations that induce pressure-driven enzyme activation. These results highlight the power of flexibility–function analyses in protein engineering design and applications.

Keywords: Biocatalysis; Computational biophysics; Enzyme mechanisms; Solution-state NMR; Transferases

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Biochemistry, Molecular Biology

Nicolas Doucet. Enzyme catalysis under pressure. Nature Catalysis, 2019, 2 (8), pp.646-647. ⟨10.1038/s41929-019-0326-3⟩. ⟨pasteur-02275285⟩