Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016 - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2019

Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016

(1, 2, 3) , (1) , (1) , (1) , (1) , (3) , (3) , (4) , (4) , (4) , (1, 5) , (3) , (3) , (2) , (1) , (6) , (3) , (1) , (1, 7)
1
2
3
4
5
6
7

Abstract

In Cambodia, highly pathogenic avian influenza A(H5N1) subtype viruses circulate endemically causing poultry outbreaks and zoonotic human cases. To investigate the genomic diversity and development of endemicity of the predominantly circulating clade 2.3.2.1c A (H5N1) viruses, we characterised 68 AIVs detected in poultry, the environment and from a single human A(H5N1) case from January 2014 to December 2016. Full genomes were generated for 42 A(H5N1) viruses. Phylogenetic analysis shows that five clade 2.3.2.1c genotypes, designated KH1 to KH5, were circulating in Cambodia during this period. The genotypes arose through multiple reassortment events with the neuraminidase (NA) and internal genes belonging to H5N1 clade 2.3.2.1a, clade 2.3.2.1b or A(H9N2) lineages. Phylogenies suggest that the Cambodian AIVs were derived from viruses circulating between Cambodian and Vietnamese poultry. Molecular analyses show that these viruses contained the hemagglutinin (HA) gene substitutions D94N, S133A, S155N, T156A, T188I and K189R known to increase binding to the human-type α2,6-linked sialic acid receptors. Two A (H5N1) viruses displayed the M2 gene S31N or A30T substitutions indicative of adamantane resistance, however, susceptibility testing towards neuraminidase inhibitors (oseltamivir, zanamivir, lananmivir and peramivir) of a subset of thirty clade 2.3.2.1c viruses showed susceptibility to all four drugs. This study shows that A(H5N1) viruses continue to reassort with other A(H5N1) and A(H9N2) viruses that are endemic in the region, highlighting the risk of introduction and emergence of novel A(H5N1) genotypes in Cambodia.
Fichier principal
Vignette du fichier
Suttie et al. - 2019 - Diversity of A(H5N1) clade 2.3.2.1c avian influenz.pdf (3.04 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

pasteur-03214760 , version 1 (02-05-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Annika Suttie, Songha Tok, Sokhoun Yann, Ponnarath Keo, Srey Viseth Horm, et al.. Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. PLoS ONE, 2019, 14 (12), pp.e0226108. ⟨10.1371/journal.pone.0226108⟩. ⟨pasteur-03214760⟩

Collections

RIIP RIIP_CAMBODGE
21 View
18 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More