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Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia
Jessica Manning
1, *
Jennifer Bohl
1
Sreyngim Lay
1
Sophana Chea
1
Ly Sovann
2
Yi Sengdoeurn
2
Seng Heng
2
Chan Vuthy
2
Katrina Kalantar
3
Vida Ahyong
4
Michelle Tan
4
Jonathan Sheu
3
Cristina Tato
4
Joseph Derisi
4
Laurence Baril
5
Veasna Duong
6
Philippe Dussart
6
Erik Karlsson
6, *
*
Corresponding author
Jessica E. Manning 1
Correspondent author
Jennifer A. Bohl 1
Author
Sreyngim Lay 1
Author
Sophana Chea 1
Author
Philippe Dussart 6
Author IdHAL : philippe-dussart ORCID : https://orcid.org/0000-0002-1931-3037
Erik A. Karlsson 6
Correspondent author
1
NIAID - National Institute of Allergy and Infectious Diseases [Phnom Penh, Cambodia] (Phnom Penh - Cambodia)
- NIAID-NIH - National Institute of Allergy and Infectious Diseases [Bethesda] (5601 Fishers Lane, Suite 6D, MSC 9804 - Bethesda, MD 20892-9804 - United States)
- NIH - National Institutes of Health [Bethesda] (9000 Rockville Pike, Bethesda, Maryland 20892 - United States)
2
Ministry of Health [Phnom Penh] (No 151-153 Kampuchea Krom Blvd. Phnom Penh - Cambodia)
3
CZI - Chan Zuckerberg Initiative [Redwood City, CA] (Redwood City, California - United States)
4
Chan Zuckerberg BioHub [San Francisco, CA] (CA 94158
San Francisco - United States)
5
Institut Pasteur du Cambodge (BP 983 - 5 , Boulevard Monivong, Phnom Penh - Cambodia)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
6
Unité de Virologie / Virology Unit [Phnom Penh] (Cambodia)
- Institut Pasteur du Cambodge (BP 983 - 5 , Boulevard Monivong, Phnom Penh - Cambodia)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
Abstract : Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation sequencing on an iSeq100 Illumina platform paired with an open-source bioinformatics pipeline to quickly characterize Cambodia's first case of COVID-2019.
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https://hal-riip.archives-ouvertes.fr/pasteur-03221716
Contributor : Philippe Dussart <>
Submitted on : Sunday, May 9, 2021 - 5:00:00 PM
Last modification on : Tuesday, June 8, 2021 - 5:29:33 PM
Contributor : Philippe Dussart <>
Submitted on : Sunday, May 9, 2021 - 5:00:00 PM
Last modification on : Tuesday, June 8, 2021 - 5:29:33 PM
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Manning et al. - 2020 - Rapid ...
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- HAL Id : pasteur-03221716, version 1
- BIORXIV : 2020.03.02.968818
- DOI : 10.1101/2020.03.02.968818
- PUBMED : 32511296
- PUBMEDCENTRAL : PMC7217139
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Jessica Manning, Jennifer Bohl, Sreyngim Lay, Sophana Chea, Ly Sovann, et al.. Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia. 2021. ⟨pasteur-03221716⟩
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