MLVA-typing on Streptococcus pneumoniae serotype 1 isolated from meningitis cases in Niger before the introduction of PCV-13 revealed a low genetic diversity

Abdelkader Alio Sanda (1) , Alexandra Granger Farbos (2) , Amina Amadou Hamidou (1) , Pascale Vonaesch (1) , Jean-François Jusot (1) , Jean-Louis Koeck (2) , Jean-Marc Collard (1)

1 CERMES - Centre de Recherche Médicale et Sanitaire (Niamey, Niger)
2 Hôpital d'Instruction des Armées Robert Picqué

Background Serotype 1 was the most prevalent pneumococcal meningitis serotype encountered in Niger over the period 2003–2011 (pre-vaccination era), accounting for 45.3% of infections. Methods Multiple locus VNTR analysis (MLVA) was used to create a genotypic snapshot of a representative subset of the pneumococcal population of serotype 1. Results MLVA using 16 markers revealed a homogeneous genetic background of pneumococci serotype 1 from Niger, which clustered with few serotype 1 pneumococci from some African countries, while other African countries displayed different clonal complexes. DNA from Niger and from other African countries were different from pneumococci serotype 1 from European countries. Conclusions MLVA-typing revealed a low genetic diversity among pneumococci serotype 1 from meningitis cases in Niger in the pre-vaccination era.

Mots clés: Genetic diversity; Multiple locus VNTR analysis; Niger; Serotype 1; Streptococcus pneumoniae

Domaines: Maladies infectieuses; Bactériologie; Santé publique et épidémiologie

Abdelkader Alio Sanda, Alexandra Granger Farbos, Amina Amadou Hamidou, Pascale Vonaesch, Jean-François Jusot, et al.. MLVA-typing on Streptococcus pneumoniae serotype 1 isolated from meningitis cases in Niger before the introduction of PCV-13 revealed a low genetic diversity. Transactions of The Royal Society of Tropical Medicine and Hygiene, 2015, 109 (7), pp.477-480. ⟨10.1093/trstmh/trv033⟩. ⟨pasteur-03238518⟩